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  5. Invitrogen™ Human PGRPs/PGLYRP1 ELISA Kit

Invitrogen™ Human PGRPs/PGLYRP1 ELISA Kit

Catalog No. EHPGLYRP1
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96 Tests
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Catalog No. Quantity
EHPGLYRP1 96 Tests
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Description

ELISA

The Human PGRPs/PGLYRP1 ELISA quantitates Hu PGRPs in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PGRPs. Principle of the method The Human PGRPs solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria (1,2) and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRP-S, PGRP-Ia, and PGRP-Ib) define a new family of human pattern recognition molecules (3). PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood (4). PGRP-S is present in neutrophils and inhibits growth of Gram-positive bacteria and, therefore, may function as a neutrophil antibacterial protein (5). However, PGRP-S may have another, as yet unidentified function because in humans it is expressed in the bone marrow 50-100 times higher than in neutrophils.

Specifications

Accession Number O75594
Assay Range 6.14-1500 pg/mL
Assay Sensitivity 6 pg/mL
Calibration Range 6 pg/mL to 1,500 pg/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA pair antibody detects human PGRPs, less than 0.05% cross-reactivity with human PGRP-1 beta and mouse PGRPs is observed.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias Cytokine tag7; MGC126894; MGC126896; peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; Pglyrp; Pglyrp1; PGRP; PGRPS; PGRP-S; SBBI68; Tag7; Tasg7; TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein); TNFSF3L; UNQ639/PRO1269Cytokine tag7; MGC126894; MGC126896; peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; Pglyrp; Pglyrp1; PGRP; PGRPS; PGRP-S; SBBI68; Tag7; Tasg7; TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein); TNFSF3L; UNQ639/PRO1269;
Gene ID (Entrez) 8993
Gene Symbol PGLYRP1
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases Peptidoglycan recognition protein short, PGRP-S
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Inflammation
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym SBBI68, PGLYRP, PGRP, PGRP-S, PGRPS, TAG7, TNFSF3L
Target PGLYRP1
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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