missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Réactifs et kits d’immunoessais
  4. Kits ELISA de recherche en sciences de la vie
  5. Invitrogen™ Mouse PAI-1 (SERPINE1) ELISA Kit

Invitrogen™ Mouse PAI-1 (SERPINE1) ELISA Kit

Numéro de catalogue. EMSERPINE1
Encompass
Modifier l'affichage
Click to view available options
Quantity:
96 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. Quantity
EMSERPINE1 96 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Numéro de catalogue. EMSERPINE1 Fournisseur Invitrogen™ Code fournisseur EMSERPINE1

Description

ELISA

The Mouse PAI-1 (SERPINE1) ELISA quantitates Ms PAI-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms PAI-1. Principle of the method The Mouse PAI-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

Spécifications

Accession Number P05121, P22777
Assay Range 80-20000 pg/mL
Assay Sensitivity 80 pg/mL
Calibration Range 80 pg/mL to 20,000 pg/mL
Content And Storage Pre-coated 96-well plate, standard, detector antibody, streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers. May be stored at 2–8°C for up to 6 months from the date of shipment.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Proteases Peptidases & Inhibitors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA pair antibody detects mouse and human PAI-1.
Sufficient For 96 Assays
Target Species Human, Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias Endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; PAI; PAI1; PAI-1; PAI1 protein; PAI1A; Pai1aa; Planh; Planh1; plas; plasminogen activator inhibitor; plasminogen activator inhibitor 1; plasminogen activator inhibitor I; plasminogen activator inhibitor, type I; plasminogen activator inhibitor-1; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI); putative plasminogen activator inhibitor-1; RATPAI1A; serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, member 1; serpin E1; serpin family E member 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE1; SERPINE-1Endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; PAI; PAI1; PAI-1; PAI1 protein; PAI1A; Pai1aa; Planh; Planh1; plas; plasminogen activator inhibitor; plasminogen activator inhibitor 1; plasminogen activator inhibitor I; plasminogen activator inhibitor, type I; plasminogen activator inhibitor-1; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI); putative plasminogen activator inhibitor-1; RATPAI1A; serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, member 1; serpin E1; serpin family E member 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE1; SERPINE-1;
Gene ID (Entrez) 18787, 5054
Gene Symbol Serpine1
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Angiogenesis
Sample Volume Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym PAI-1, PAI1, Planh1
Target PAI1
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
Afficher plus de résultats Afficher moins de résultats