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Gibco™ Opti-MEM™ I Reduced Serum Medium
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Catalog No. 31985070
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Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.

This Opti-MEM™ I is modified as follows:

With: L-glutamine, Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM™ I Medium

Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

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Shipping Condition: Room Temperature

Specifications

Cell Line Sp2, AE-1, CHO, BHK-21, and HEK
Cell Type Primary Fibroblasts
Classification Human Origin
Concentration 1 X
Form Liquid
Product Type Opti-MEM I
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, Phenol Red
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco, Opti-MEM
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Store at 2–8°C and protect from light.
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.