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  5. Invitrogen™ Mouse IFN gamma ELISA Kit

Invitrogen™ Mouse IFN gamma ELISA Kit

Numéro de catalogue. KMC4021
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Quantité:
2 x 96 Tests
5 x 96 Tests
96 Tests
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Numéro de catalogue. Quantité
KMC4021 96 Tests
KMC4022 2 x 96 Tests
KMC4021C 5 x 96 Tests
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Numéro de catalogue. KMC4021 Fournisseur Invitrogen™ Code fournisseur KMC4021

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Description

Comprend

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Mouse Interferon Gamma (Ms IFNγ) ELISA quantitates Ms IFNγ in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IFNγ. Principle of the method The Mouse IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Order Info

Shipping Condition: Room Temperature

Spécifications

Numéro d'enregistrement P01580
Plage de l'essai 47-300 pg/mL
Sensibilité d’essai <2 pg/mL
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Cytokines & Receptors
Type d’échantillon Plasma, Serum, Supernatant
Espèces cibles Mouse
À utiliser avec (application) Colorimetric Detection
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-gBoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g; IFN-g; Ifg
ID du gène (Entrez) 15978
Symbole de gène IFNG
Temps d’incubation 4 hr.
CV intra-essai 6.5%, 3.2%
Contenus des kits Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant Biotin
Marqueur génétique IFN-γ
Alias des protéines IFN-gamma
Quantité 96 Tests
Statut réglementaire RUO
Volume d’échantillon Plasma 25 μL, Serum 25 μL, Supernatant 25 μL
Shipping condition (Condition d'expédition) Wet Ice
Conditions de stockage 2-8°C
Cible IFN gamma
Durée du test 1 hr 20 min
Durée totale de l'essai 4 hr
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For Research Use Only. Not for use in diagnostic procedures.