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Invitrogen™ Mouse Ferritin ELISA Kit

Numéro de catalogue. EEL095
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Quantité:
96 Tests
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Numéro de catalogue. Quantité
EEL095 96 Tests
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Numéro de catalogue. EEL095 Fournisseur Invitrogen™ Code fournisseur EEL095

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Description

ELISA

The Mouse Ferritin ELISA quantitates Ferritin in serum, plasma, and other biological fluids The Mouse Fibronectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.

Spécifications

Numéro d'enregistrement P09528, P29391
Plage de l'essai 1.56-100 ng/mL
Sensibilité d’essai 0.94 ng/mL
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Ferritin
Type d’échantillon Plasma, Serum
Espèces cibles Mouse
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène apoferritin; cell proliferation-inducing gene 15 protein; Ferritin H subunit; ferritin heavy chain; ferritin heavy chain 1; Ferritin heavy chain, N-terminally processed; Ferritin L subunit; ferritin L-chain; ferritin light chain; ferritin light polypeptide-like 3; Ferritin subunit H; ferritin, heavy polypeptide 1; ferritin, light polypeptide; FHC; frih; fril; FTH; Fth1; FTHL6; FTL; HFE5; H-ferritin; HFt; L apoferritin; LFTD; MFH; MGC71996; NBIA3; OK/SW-cl.84; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15apoferritin; cell proliferation-inducing gene 15 protein; Ferritin H subunit; ferritin heavy chain; ferritin heavy chain 1; Ferritin heavy chain, N-terminally processed; Ferritin L subunit; ferritin L-chain; ferritin light chain; ferritin light polypeptide-like 3; Ferritin subunit H; ferritin, heavy polypeptide 1; ferritin, light polypeptide; FHC; frih; fril; FTH; Fth1; FTHL6; FTL; HFE5; H-ferritin; HFt; L apoferritin; LFTD; MFH; MGC71996; NBIA3; OK/SW-cl.84; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15;
ID du gène (Entrez) 14319, 14325
Symbole de gène FTH1, Ftl1
CV intra-essai <10%
Contenus des kits Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Étiquette ou colorant Biotin
Quantité 96 Tests
Statut réglementaire RUO
Volume d’échantillon Plasma 100 μL, Serum 100 μL
Shipping condition (Condition d'expédition) Wet or Dry Ice
Conditions de stockage Refer to product documentation for component specific storage temperature.
Cible Ferritin
Durée du test 1 hr 20 min
Durée totale de l'essai 3 hr 30 min
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