missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Réactifs et kits d’immunoessais
  4. Kits ELISA de recherche en sciences de la vie
  5. Invitrogen™ Human Luteinizing Hormone ELISA Kit

Invitrogen™ Human Luteinizing Hormone ELISA Kit

Numéro de catalogue. EHLH
Encompass
Modifier l'affichage
Click to view available options
Quantité:
96 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. Quantité
EHLH 96 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Numéro de catalogue. EHLH Fournisseur Invitrogen™ Code fournisseur EHLH

Description

ELISA

The Human Luteinizing Hormone (Hu LH) ELISA quantitates Hu LH in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu LH. Principle of the method The Human LH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

LH is a member of the glycoprotein hormone family, Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.

Spécifications

Numéro d'enregistrement P01229
Plage de l'essai 156.3 to 10,000 pg/mL
Sensibilité d’essai 140 pg/mL
Plage d’étalonnage 140 pg/mL to 10,000 pg/mL
Essai Solid-phase sandwich ELISA
Conjugué HRP
Type de produit ELISA
Famille de protéines Other Proteins
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Suffisant pour 96 assays
Espèces cibles Human
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Colorimetric Microplate Reader
ID du gène (Entrez) 1081, 3972
Symbole de gène CGB4,HH23,LSH-B,LSH-beta
CV intra-essai <12%, <10
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant HRP
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Signal Transduction
Volume d’échantillon Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Conditions de stockage 2°C to 8°C
Synonyme RP11-156F23.1, CG-ALPHA, FSHA, GPHA1, GPHa, HCG, LHA, TSHA
Cible Luteinizing Hormone
Durée du test 1 hrs 20 mins
Durée totale de l'essai 4 hrs 45 mins
Afficher plus de résultats Afficher moins de résultats