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  5. Invitrogen™ Human LDLR ELISA Kit

Invitrogen™ Human LDLR ELISA Kit

Numéro de catalogue. EHLDLR
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96 Tests
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Numéro de catalogue. Quantité
EHLDLR 96 Tests
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Numéro de catalogue. EHLDLR Fournisseur Invitrogen™ Code fournisseur EHLDLR

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Description

ELISA

The Human LDLR ELISA quantitates Hu LDLR in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu LDLR. Principle of the method The Human LDLR solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.

Spécifications

Numéro d'enregistrement P01130
Plage de l'essai 8.19-2000 pg/mL
Sensibilité d’essai 8 pg/mL
Plage d’étalonnage 8 pg/mL to 2,000 pg/mL
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Essai Solid-phase sandwich ELISA
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Other Proteins
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA pair antibody detects human LDLR. Other species not determined yet.
Suffisant pour 96 assays
Espèces cibles Human
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène FH; FHC; Hlb301; I79_005860; LDL receptor; LDLA; LDLCQ2; LDLR; ldl-r; LDLRA; low density lipoprotein receptor; low density lipoprotein receptor protein; low-density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3FH; FHC; Hlb301; I79_005860; LDL receptor; LDLA; LDLCQ2; LDLR; ldl-r; LDLRA; low density lipoprotein receptor; low density lipoprotein receptor protein; low-density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; FH; FHC; LDLCQ2
ID du gène (Entrez) 3949
Symbole de gène LDLR
CV intra-essai <12%, <10%
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant Biotin
Alias des protéines LDL receptor
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Virology
Volume d’échantillon Plasma 2.5 μL, Serum 2.5 μL, Supernatant 100 μL
Conditions de stockage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonyme FH, FHC, LDLCQ2
Cible LDLR
Durée du test 1 hr 20 min
Durée totale de l'essai 4 hr 45 min
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