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Invitrogen™ Dynabeads™ Human DC Enrichment Kit 
Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes.
Supplier: Invitrogen™ 11308D
Comprend: 2x10mL Depletion MyOne; Streptavidin Dynabeads and 4mL Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards CD3, CD14, CD16, CD19, CD56 and Glycophorin A.
Description
Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes. Highly enriched DC's are bead and antibody-free, and can be used in any functional assay. The cells should be further sorted to obtain high purity of the specific DC sub-populations of choice.
• Obtain high recovery (80-100%) of viable DC's of all sup-populations.
• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.
Methodology:
An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC's can be directly sorted into the sup-population of choice or used in any other application.
Starting samples:
PBMC
Order Info
Shipping Condition: Room Temperature
Spécifications
| Dendritic Cells | |
| Cell Enrichment Kit | |
| Processes ∼2 x 109 cells total | |
| DYNAL, Dynabeads | |
| 2 x 10 mL | |
| Room Temperature | |
| Human |
| The Kit Contains: 2 x 10 mL Depletion MyOne™ Streptavidin Dynabeads™ and 4 mL Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards CD3, CD14, CD16, CD19, CD56 and Glycophorin A. Store at 2°C to 8°C. |
|
| Negative Isolation | |
| >95% | |
| Research Grade | |
| PBMC | |
| 1 x 107 PBMCs per isolation |
For Research Use Only. Not for use in diagnostic procedures.