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  5. Invitrogen™ Human IL-28A ELISA Kit

Invitrogen™ Human IL-28A ELISA Kit

Numéro de catalogue. EHIL28A
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96 Tests
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Numéro de catalogue. Quantité
EHIL28A 96 Tests
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Numéro de catalogue. EHIL28A Fournisseur Invitrogen™ Code fournisseur EHIL28A

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Description

ELISA

The Human Interleukin 28A (Hu IL-28A) ELISA quantitates Hu IL-28A in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-28A. Principle of the method The Human IL-28A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role.

Spécifications

Numéro d'enregistrement Q8IZJ0
Plage de l'essai 15-10000 pg/mL
Sensibilité d’essai 15 pg/mL
Plage d’étalonnage 15 pg/mL to 10000 pg/mL
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Essai Solid-phase sandwich ELISA
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Other Proteins
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA kit shows no cross-reactivity with any of the cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-16, IL-17, IL-18, IL-29, IL-309, IP-10, GCSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Suffisant pour 96 assays
Espèces cibles Human
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène cytokine Zcyto20; EG330496; Ifnl2; IFN-lambda2; IFN-lambda-2; Il28a; IL-28A; ILN; interferon lambda 2; interferon lambda-2; interferon, lambda 2; interferon-lambda 2; interferon-lambda2; Interleukin; interleukin 28A; interleukin 28A (interferon, lambda 2); interleukin-28A; ZCYTO20cytokine Zcyto20; EG330496; Ifnl2; IFN-lambda2; IFN-lambda-2; Il28a; IL-28A; ILN; interferon lambda 2; interferon lambda-2; interferon, lambda 2; interferon-lambda 2; interferon-lambda2; Interleukin; interleukin 28A; interleukin 28A (interferon, lambda 2); interleukin-28A; ZCYTO20; IL-28A; IL28A
ID du gène (Entrez) 282616
Symbole de gène IFNL2
CV intra-essai <12%, <10%
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant Biotin
Alias des protéines IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Virology
Volume d’échantillon Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Shipping condition (Condition d'expédition) Wet or Dry Ice
Conditions de stockage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonyme IL-28 A, IL28A
Cible IL-28A
Durée du test 1 hr 20 min
Durée totale de l'essai 4 hr 45 min
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