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  5. Invitrogen™ Mouse IL-2 Uncoated ELISA Kit

Invitrogen™ Mouse IL-2 Uncoated ELISA Kit

Numéro de catalogue. 5017190
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10 x 96 Tests
20 x 96 Tests
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Numéro de catalogue. Quantité
5017190 20 x 96 Tests
5017192 10 x 96 Tests
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Numéro de catalogue. 5017190 Fournisseur Invitrogen™ Code fournisseur 88702477

Description

ELISA

The Mouse Interleukin-2 (IL-2) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-2. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Spécifications

Numéro d'enregistrement P04351
Plage de l'essai 2 to 200 pg/mL
Sensibilité d’essai 2 pg/mL
Conjugué HRP
Type de produit ELISA
Type d’échantillon Serum, Supernatant
Espèces cibles Mouse
À utiliser avec (application) ELISA
À utiliser avec (équipement) Colorimetric Microplate Reader
ID du gène (Entrez) 16183
Symbole de gène DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
Contenus des kits Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Étiquette ou colorant HRP
Quantité 20 x 96 Tests
Statut réglementaire RUO
Volume d’échantillon Serum 100 μL, Supernatant 100 μL
Conditions de stockage 2°C to 8°C
Synonyme Interleukin-2
Cible IL-2
Durée du test 1 hrs 45 mins
Durée totale de l'essai 24 hrs 30 mins
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