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Invitrogen™ Human IL-17A ELISA Kit

Numéro de catalogue. KAC1591
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96 Tests
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Numéro de catalogue. Quantité
KAC1591 96 Tests
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Numéro de catalogue. KAC1591 Fournisseur Invitrogen™ Code fournisseur KAC1591

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Description

ELISA

The Human Interleukin 17A (Hu IL-17A) ELISA quantitates Hu IL-17A in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-17A. Principle of the method The Human IL-17A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

Spécifications

Numéro d'enregistrement Q16552
Plage de l'essai 15-1000 pg/mL
Sensibilité d’essai 2 pg/mL
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Cytokines & Receptors
Type d’échantillon Plasma, Serum, Supernatant
Espèces cibles Human
À utiliser avec (application) Colorimetric
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène Ctla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-ACtla8; CTLA-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; IL17A; IL-17a; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17A; interleukin-17; Interleukin17A; interleukin-17A; R-IL-17-A; Ctla-8; Ctla8; IL-17; IL-17A; Il17
ID du gène (Entrez) 3605
Symbole de gène IL17A
CV intra-essai 3.7%
Contenus des kits Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
Étiquette ou colorant HRP
Precision 2 pg/mL
Alias des protéines IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8
Quantité 96 Tests
Statut réglementaire RUO
Volume d’échantillon Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Shipping condition (Condition d'expédition) Wet Ice
Conditions de stockage 2-8°C
Cible IL-17A
Durée du test 1 hr 20 min
Durée totale de l'essai 2 hr 45 min
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