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Invitrogen™ Mouse IgG2b Uncoated ELISA Kit
Description
The Mouse Immunoglobulin G2b (IgG2b) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IgG2b. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Spécifications
Spécifications
| Plage de l'essai | 3.90-250 ng/mL |
| Sensibilité d’essai | 3.90 ng/mL |
| Contenu et stockage | Concentré d’anticorps de captureConcentré d’anticorps de détectionContrôle isotype IgG2b de souris (standard), lyophilisé, 500 ng/mL lors de la reconstitutionConcentré de solution saline tamponnée phosphate (PBS)Tampon d’essai A 20x (PBS avec 1% Tween 20 et 10% BSA)Solution substrat tétramétylbenzidine (TMB) Plaques à 96 puits incluses avec le produit Cat. Les numéros se terminant par les suffixes -22, -76, -86 |
| Conjugué | HRP |
| Format de la trousse d’immunodosage | Kit non revêtu ELISA |
| Type de produit | ELISA |
| Type d’échantillon | Plasma, Sérum, Surnageant |
| Espèces cibles | Souris |
| À utiliser avec (application) | ELISA |
| À utiliser avec (équipement) | Lecteur de microplaques à absorbance |
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