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Gibco™ Human Endothelial SFM
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Catalog No. 11111044
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500 mL
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11111044 500 mL
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Catalog No. 11111044 Supplier Gibco™ Supplier No. 11111044

Description

Human Endothelial Serum Free Medium (SFM) has been developed to support the long-term propagation of human umbilical vein endothelial cells (HUVEC).

Human Endothelial Serum Free Medium (SFM) has been developed to support the long-term propagation of human umbilical vein endothelial cells (HUVEC). Complete Human Endothelial SFM has been demonstrated to successfully support primary isolation and subsequent secondary growth of HUVEC for ≤15 passages. HUVEC cultured in complete Human Endothelial SFM exhibit the histotypic 'cobblestone' morphology and retain endothelial specific markers including: expression of Factor VIII-related antigen, UEA-1 lectin binding, uptake of DiI-acetylated LDL, vimentin, and IL-1a-induced ICAM-1 expression. Additionally, HUVEC cultured in Human Endothelial SFM have been shown to maintain cAMP and prostacyclin signal transduction systems. Human Endothelial SFM also supports the growth and retention of physiological markers for human umbilical arterial and dermal microvascular endothelial cells.

Human Endothelial SFM is a basal serum-free growth medium and requires supplementation with bFGF (20 ng/mL), EGF (10 ng/mL), and human plasma fibronectin (10 μg/mL).

Specifications

Cell Line HUAEC, HUVEC
Cell Type Endothelial Cells
Classification cGMP, Serum-free, Chemically-defined
Form Liquid
Product Type Serum Free Medium (SFM)
Serum Level Serum-free
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, Phenol Red
Without Additives No Antibiotics
Quantity 500 mL
Shipping Condition Room Temperature
Endotoxin Level ≤10 EU/mL, ≤ 10 EU/mL
Content And Storage Store in refrigerator (2–8°C) and protect from light.
Culture Type Mammalian Cell Culture
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Frequently Asked Questions (FAQs)

Do you offer a phenol red-free option for Human Endothelial-SFM (Cat. No. 11111044)?

We do not offer a phenol red-free version of this media as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

For Research Use Only. Not for use in diagnostic procedures.