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  5. Invitrogen™ Human Cystatin A ELISA Kit

Invitrogen™ Human Cystatin A ELISA Kit

Catalog No. EH140RBX5
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5 x 96 Tests
96 Tests
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EH140RBX5 5 x 96 Tests
EH140RB 96 Tests
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Description

ELISA

Human Cystatin A quantitates human Cystatin A in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Cystatin A.

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Specifications

Accession Number P01040
Assay Range 6.5-1500 pg/mL
Assay Sensitivity 6.5 pg/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias AREI; Csta; Csta1; cystatin A; cystatin A (stefin A); cystatin A1; cystatin AS; cystatin-A; Cystatin-A, N-terminally processed; Cystatin-A1; Cystatin-AS; PSS4; Stefin A; stefin-A; STF1; STFAAREI; Csta; Csta1; cystatin A; cystatin A (stefin A); cystatin A1; cystatin AS; cystatin-A; Cystatin-A, N-terminally processed; Cystatin-A1; Cystatin-AS; PSS4; Stefin A; stefin-A; STF1; STFA;
Gene ID (Entrez) 1475
Gene Symbol CSTA
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target Cystatin A
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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