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  5. Invitrogen™ Human Cystatin A ELISA Kit

Invitrogen™ Human Cystatin A ELISA Kit

Catalog No. EH140RBX5
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5 x 96 Tests
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EH140RBX5 5 x 96 Tests
EH140RB 96 Tests
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Description

ELISA

Human Cystatin A quantitates human Cystatin A in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Cystatin A.

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Specifications

Accession Number AREI; Csta; Csta1; cystatin A; cystatin A (stefin A); cystatin A1; cystatin AS; cystatin-A; Cystatin-A, N-terminally processed; Cystatin-A1; Cystatin-AS; PSS4; Stefin A; Stefin-A; STF1; STFA
Assay Range 6.5 to 1,500 pg/mL
Assay Sensitivity 6.5 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1475
Gene Symbol CSTA
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Cystatin A
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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