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  7. Gibco™ Sf-900™ III SFM

Gibco™ Sf-900™ III SFM

Catalog No. 12658035
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Catalog No. 12658035 Supplier Gibco™ Supplier No. 12658035
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Description

A low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium

Sf-900 III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells.

  • Optimized for recombinant gene expression using the baculovirus and stable insect expression systems
  • Suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines
  • Spodoptera frugiperda (Sf9) cells grown in Sf-900 III SFM achieve maximum cell densities of 10–14 x 106 cells/mL
  • An improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins
  • Ready to use format does not require addition of serum, glutamine, or surfactants
  • Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900 III SFM
  • Reduced hydrolysate concentrations improve lot-to-lot consistency

Order Info

Shipping Condition: Room temperature

Compliance

Gibco Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Cell Line Sf21, Sf9
Cell Type Insect Cell
Classification Animal Origin-free, Protein-free, Serum-free
Form Liquid
Product Type Insect Cell Serum Free Medium (SFM)
Serum Level Serum-free
With Additives Glutamine
Product Line Gibco, Sf-900
Quantity 10 L
Shipping Condition Room Temperature
Species S. frugiperda, Spodoptera frugiperda
Content And Storage Storage conditions: 2–8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture
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Frequently Asked Questions (FAQs)

What antimicrobials can be used in insect culture and at what concentration?

Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)

Is it necessary to heat-inactivate my serum before adding it to my medium?

Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.

Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.

Do you offer serum-free insect media?

Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.
Will your company continue to support other films?

Yes. Although we feel that the PE film exceeds many of the technical properties of the other available films, we will continue to offer and support our other currently available films through custom orders.

In what size package will my Gibco Cell Culture Bags ship?

Each bag size will arrive in a specific heavyweight corrugated box designed for shipment:
5 L: 12” x 14.25” x 5.5”
10 L: 13” x 23.5” x 6.125”
20 L: 15” x 24” x 6.125”

What are the benefits of the Gibco PE film?

The commercially available film is a very clear, multi-layered, single web film with a polyethylene contact surface that can be made into a wide range of bag sizes (250 mL to 1,000L). In addition, the film is a market leader with exceptional gas barrier properties and very low extractable and leachable results. Click here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).
What is the Gibco PE Film?

Based on feedback from customers, we designed a media bag portfolio that uses a consistent film for all bag sizes. This film, called PE film, is superior in function to other commercially available films. Please go here to read more about the PE Film specifications (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).
What are Gibco Cell Culture Bags?

Gibco Cell Culture Bags are convenient, pre-filled bags in a variety of formulations and sizes, specifically designed to increase productivity and reduce costs. The bags can be formatted up to 1,000 L. Read more about these bags here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/gibco-cell-culture-bags.html).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.