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Gibco™ RPMI 1640 Medium (ATCC modification) DFS Item

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Supplier:  Gibco™ A1049101

Catalog No. A1049101

Requires : Supplementation



RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
With: L-glutamine, HEPES, Phenol Red, Sodium Pyruvate, High glucose, Low sodium bicarbonate

The complete formulation is available.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. This RPMI formulation has been modified by ATCC to contain high glucose, low sodium bicarbonate, sodium pyruvate, HEPES, and low L-glutamine. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (1.5 g/L) and therefore requires a 5–7% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature


RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.



Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Leukemic Cells
1 X
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sustainable packaging
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Animal Origin-free
Standard Serum Supplementation
High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate, Low Sodium Bicarbonate
cGMP-compliant under the ISO 13485 standard
Room Temperature




For Research Use Only. Not for use in diagnostic procedures.