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Gibco™ IMDM, powder 
Iscove's Modified Dulbecco's Medium is well suited for rapidly proliferating, high-density cell cultures including Jurkat, COS-7, and macrophage cells
Manufacturer: Gibco™ 12200036
Requires : Supplementation
Description
- Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
- Store at 2°C to 8°C
Modified as follows:
- With: L-glutamine, phenol red
- Without: α-thioglycerol, 2-mercaptoethanol
Using IMDM
- IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins
- In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate
- It contains no proteins, lipids, or growth factors therefore IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
- Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation
Specifications
| IMDM (Iscove's Modified Dulbecco's Medium) | |
| Room Temperature | |
| Powder | |
| Phenol Red | |
| 10 x 1 L | |
| α-Thioglycerol, 2-Mercaptoethanol | |
| Sodium Pyruvate | |
| Animal Origin-Free | |
| HEPES |
| Sterile-filtered | |
| Gibco™ | |
| L-Glutamine | |
| 36 Months From Date of Manufacture | |
| Phenol Red, L-Glutamine | |
| High Glucose | |
| Jurkat, COS-7, and macrophage cells | |
| IMDM (Iscove's Modified Dulbecco's Medium) |
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.