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  7. Gibco™ Grace's Insect Medium, supplemented

Gibco™ Grace's Insect Medium, supplemented
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Catalog No. 11605094
Encompass
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500 mL
10 x 500 mL
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11605094 500 mL
11605102 10 x 500 mL
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Catalog No. 11605094 Supplier Gibco™ Supplier No. 11605094
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Description

Requires

Grace's Unsupplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.

Widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21

Originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS).

  • Contains lactalbumin hydrolysate, yeastolate, L-glutamine, and sodium bicarbonate
  • Dual site cGMP manufacturing and quality system
  • Commonly supplemented with 10% Fetal Bovine Serum (FBS) before use

Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture

Order Info

Shipping Condition: Room temperature

Specifications

Cell Line Sf21, Sf9
Cell Type Insect Cell
Form Liquid
Product Type Grace's Insect Medium
With Additives Glutamine, Yeastolate, Lactalbumin Hydrolysate
Without Additives No Insect Hemolymph
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Inorganic Salts Calcium, Magnesium
Content And Storage Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Frequently Asked Questions (FAQs)

For how long can I store Grace's Insect Medium, unsupplemented at room temperature?

We ship this product under “ambient” conditions throughout the year. We have performed shipping studies that shown components to be stable for at least 7 days at ambient temperatures. The important factor is to ensure that the media is not exposed to light at the warmer temperatures as there are vitamins and amino acids that will breakdown with light exposure.
What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

For Research Use Only. Not for use in diagnostic procedures.