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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/µL)

A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.

$197.00 - $806.00

Specifications

Concentration 200 U/μL
Final Product Type First-Strand cDNA
Format Reverse Transcriptase
Reaction Format Separate components
Reaction Speed 60 min.
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
FEREP0451
View Documents Promotion Details
Thermo Scientific™
EP0451
10,000 Units
Each for $197.00
 
FEREP0452
View Documents Promotion Details
Thermo Scientific™
EP0452
5 x 10,000 Units
Each for $806.00
 
Description

Description

RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).

Highlights

  • High yields of full-length first strand cDNA up to 13 kb
  • Optimum activity at 42 to 45°C
  • Active up to 55°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Specifications

Specifications

200 U/μL
Reverse Transcriptase
60 min.
Up to 13 kb
42°C to 45°C
Reduced
First-Strand cDNA
Separate components
Reverse Transcription
RNA
RevertAid H Minus
SDS
Product Certifications