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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase
A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.
$129.00 - $516.00
Specifications
| For Use With (Application) | First strand cDNA synthesis for RT-PCR and real-time RT-PCR (3, 4, 5); Reverse transcription at elevated temperatures to reduce effects of secondary structure; Synthesis of cDNA for cloning and expression |
|---|---|
| Product Name | RevertAid H Minus Reverse Transcriptase |
Description
RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).
Highlights
- High yields of full-length first strand cDNA up to 13 kb
- Optimum activity at 42 to 45°C
- Active up to 55°C
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Specifications
| First strand cDNA synthesis for RT-PCR and real-time RT-PCR (3, 4, 5); Reverse transcription at elevated temperatures to reduce effects of secondary structure; Synthesis of cDNA for cloning and expression | |
| RevertAid H Minus Reverse Transcriptase |