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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/µL)
A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.
$197.00 - $806.00
Specifications
| Concentration | 200 U/μL |
|---|---|
| Final Product Type | First-Strand cDNA |
| Format | Reverse Transcriptase |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
Description
RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).
Highlights
- High yields of full-length first strand cDNA up to 13 kb
- Optimum activity at 42 to 45°C
- Active up to 55°C
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Specifications
| 200 U/μL | |
| Reverse Transcriptase | |
| 60 min. | |
| Up to 13 kb | |
| 42°C to 45°C | |
| Reduced |
| First-Strand cDNA | |
| Separate components | |
| Reverse Transcription | |
| RNA | |
| RevertAid H Minus |