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  5. Invitrogen™ Mouse DAN (PARN) ELISA Kit

Invitrogen™ Mouse DAN (PARN) ELISA Kit

Numéro de catalogue. EMPARN
Encompass
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96 Tests
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Numéro de catalogue. Quantité
EMPARN 96 Tests
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Numéro de catalogue. EMPARN Fournisseur Invitrogen™ Code fournisseur EMPARN

Description

ELISA

The Mouse DAN (PARN) ELISA quantitates Ms DAN in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms DAN. Principle of the method The Mouse DAN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Spécifications

Numéro d'enregistrement Q8VDG3
Plage de l'essai 40.96 to 10,000 pg/mL
Sensibilité d’essai 35 pg/mL
Plage d’étalonnage 35 pg/mL to 10,000 pg/mL
Essai Solid-phase sandwich ELISA
Conjugué HRP
Type de produit ELISA
Famille de protéines Other Proteins
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA pair antibody detects mouse DAN, no cross-reactivity with rhDAN, rhCOCO, rmBMP-4 or rmGremlin
Suffisant pour 96 assays
Espèces cibles Mouse
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Colorimetric Microplate Reader
ID du gène (Entrez) 74108
Symbole de gène 1200003I18Rik,DAN
CV intra-essai <12%, <10
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant HRP
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Other Research
Volume d’échantillon Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Conditions de stockage 2°C to 8°C
Synonyme 1200003I18Rik, DAN
Cible PARN
Durée du test 1 hrs 20 mins
Durée totale de l'essai 4 hrs 45 mins
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