missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Réactifs et kits d’immunoessais
  4. Kits ELISA de recherche en sciences de la vie
  5. Invitrogen™ Mouse CTLA-4 ELISA Kit

Invitrogen™ Mouse CTLA-4 ELISA Kit

Numéro de catalogue. EMCTLA4
Encompass
Modifier l'affichage
Click to view available options
Quantité:
96 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. Quantité
EMCTLA4 96 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Numéro de catalogue. EMCTLA4 Fournisseur Invitrogen™ Code fournisseur EMCTLA4

Description

ELISA

The Mouse CTLA-4 ELISA quantitates Ms CTLA-4 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CTLA-4. Principle of the method The Mouse CTLA-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

Spécifications

Numéro d'enregistrement P09793
Plage de l'essai 5-1200 pg/mL
Sensibilité d’essai 5 pg/mL
Plage d’étalonnage 5 pg/mL to 1200 pg/mL
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Aperçu général CTLA-4 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Chemokines and Receptors Protein Family, Immunology Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Ctla-4 in Samples, Using a Microplate Reader
Analytes détectables CTLA-4
Essai Solid-phase sandwich ELISA
Conjugué HRP
Méthode de détection Colorimetric
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Chemokines & Receptors
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL- 17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Type de substrat TMB
Suffisant pour 96 Assays
Espèces cibles Mouse
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène ALPS5; CD; CD152; CD152 antigen; CD152 isoform; CD152 protein; CD152 protein precursor; celiac disease 3; CELIAC3; Ctla4; CTLA-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; cytotoxic T-lymphocyte associated protein 4; cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; EGK_04712; GRD4; GSE; ICOS; IDDM12; insulin-dependent diabetes mellitus 12; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; Ly-56; sCTLA4; soluble form; transmembrane formALPS5; CD; CD152; CD152 antigen; CD152 isoform; CD152 protein; CD152 protein precursor; celiac disease 3; CELIAC3; Ctla4; CTLA-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; cytotoxic T-lymphocyte associated protein 4; cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; EGK_04712; GRD4; GSE; ICOS; IDDM12; insulin-dependent diabetes mellitus 12; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; Ly-56; sCTLA4; soluble form; transmembrane form; Cd152; Ctla-4; Ly-56
ID du gène (Entrez) 12477
Symbole de gène CTLA4
Espèces hôtes Mouse
CV intra-essai <12%, <10%
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant Biotin
Alias des protéines Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Immunology
Volume d’échantillon Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Shipping condition (Condition d'expédition) Wet or Dry Ice
Conditions de stockage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonyme CH29-120A16.2, Cd152, Ctla-4, Ly-56
Cible CTLA-4 (CD152)
Durée du test 1 hr 20 min
Durée totale de l'essai 4 hr 45 min
Afficher plus de résultats Afficher moins de résultats