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  5. Invitrogen™ Rat CNTF ELISA Kit

Invitrogen™ Rat CNTF ELISA Kit

Numéro de catalogue. ERCNTF
Encompass
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96 Tests
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Numéro de catalogue. Quantité
ERCNTF 96 Tests
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Numéro de catalogue. ERCNTF Fournisseur Invitrogen™ Code fournisseur ERCNTF

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Description

ELISA

The Rat Ciliary NeuroTrophic Factor (Rt CNTF) ELISA quantitates Rt CNTF in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt CNTF. Principle of the method The Rat CNTF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.

Spécifications

Numéro d'enregistrement P20294
Plage de l'essai 10-2000 pg/mL
Sensibilité d’essai 10 pg/mL
Plage d’étalonnage 10 pg/mL to 2000 pg/mL
Contenu et stockage Pre-coated 96-well plate, standard, detector antibody, streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers. May be stored at 2–8°C for up to 6 months from the date of shipment.
Essai Solid-phase sandwich ELISA
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Growth Factors & Receptors
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP- 1, TNF-alpha, VEGF.
Suffisant pour 96 assays
Espèces cibles Rat
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène AI429687; Ciliary neurotrophic factor; ciliary neurotropic factor; Cntf; HCNTF; H-CNTFAI429687; Ciliary neurotrophic factor; ciliary neurotropic factor; Cntf; HCNTF; H-CNTF;
ID du gène (Entrez) 25707
Symbole de gène CNTF
CV intra-essai <12%, <10%
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant Biotin
Alias des protéines CNTF
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Oncology
Volume d’échantillon Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Shipping condition (Condition d'expédition) Wet or Dry Ice
Conditions de stockage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Cible CNTF
Durée du test 1 hr 20 min
Durée totale de l'essai 4 hr 45 min
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