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MilliporeSigma™ Chemicon™ Endothelial Cell Characterization Kit

Catalog No. SCR023
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SCR023 1 Kit
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Catalog No. SCR023 Supplier MilliporeSigma™ Supplier No. SCR023
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Description

Enables researchers to characterize cultured endothelial cells in both constitutive and activated state using basic Immunocytochemistry techniques.

Following antibody dilutions are recommended for immunocytochemistry:

  • Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2ng/mL
  • Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2ng/mL
  • Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20ng/mL

Following antibodies have been shown to stain cultured HUVEC after 4 to 6hr of TNF-alpha (100ng/mL) induction at following dilutions:

  • Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2ng/mL
  • Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2ng/mL

Specifications

Cell Type Mouse Embryonic Stem Cells, Human Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells, Hematopoietic Stem Cells, Epithelial Cells, Pancreatic Stem Cells, Cardiac Stem Cells, Induced Pluripotent Stem Cells
Product Type Stem Cell Characterization Kit
For Use With (Application) Immunocytochemistry
Quantity 1 Kit
Content And Storage
  • 50μg of Endothelial Cell (CD146) monoclonal (Part No. 2003789)
  • 50μg of CD31 monoclonal (Part No. 2003788)
  • 10μg of von Willibrand Factor (vWF) polyclonal (Part No. 2003787)
  • 50μg of VCAM-1 monoclonal (Part No.MAB2511-50UG)
  • 50μg of ICAM-1 monoclonal (Part No. 2003785)
  • 100μg of Mouse control IgG (Part No. PP54-100UG)
  • 100μg of Rabbit control IgG (Part No. PP64-100UG)
Antibodies to ICAM-1, VCAM-1, von Willibrand Factor (vWF), and Endothelial Cells (CD146) are to be stored at 2° to 8°C (SCR023 - Part 1). Antibodies to CD31, Mouse IgG, and Rabbit IgG are to be stored at -20°C (SCR023 - Part 2).

For Research Use Only