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Gibco™ AAV-MAX Helper-Free AAV Production System Kit

Catalog No. A51217
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Description

The AAV-MAX Helper-Free AAV Production System Kit is a scalable, high-titer platform for suspension AAV production.

The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.

Features of the AAV-MAX Helper-Free AAV Production System include:

  • Components work synergistically to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
  • Suspension-based system has scalable protocols from shake flask to bioreactor scale
  • Simplified workflow—streamlined protocol using helper virus-free triple transfection
  • Clonal, 293F-derived, high-producer cell line
  • Robust transfection protocols are adaptable to large-scale transfection
  • The components included in the AAV-MAX System Kit are:
    • 1 vial of Viral Production Cells 2.0
    • 1 L of Viral Production Medium
    • 1 AAV-MAX Transfection Kit for 1 L of production
    • 100 mL Viral-Plex Complexation Buffer
    • 100 mL AAV-MAX Lysis Buffer
    • 100 mL GlutaMAX Supplement

All components are also available for purchase separately.

Specifications

Cell Line Viral Production Cells 2.0
Cell Type Kidney (Embryonic)
Product Type AAV Production System
Sterility Sterile
For Use With (Application) AAV Production
Product Line AAV-MAX
Quantity Sufficient for 1 L of Culture
Content And Storage • 1 vial Viral Production Cells 2.0
• 1 L Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer
• 100 mL GlutaMAX Supplement

Storage conditions vary
Culture Type Suspension Cell Culture

Frequently Asked Questions (FAQs)

Are the components in the AAV-MAX Helper-Free AAV Production System available separately, or are they only sold as a kit?

The Viral Production Medium, Viral Production Cells 2.0, AAV-MAX Transfection Kit, Viral-Plex Complexation Buffer, and AAV-MAX Lysis Buffer are sold individually and here are the stand-alone catalog numbers:
- Viral Production Medium: Cat. No. A4817901, A4817902, A4817903
- Viral Production Cells 2.0: Cat. No. A49784, A51218
- AAV-MAX Transfection Kit: Cat. No. A50515, A50516
- Viral-Plex Complexation Buffer: A4983901
- AAV-MAX Lysis Buffer: A50520

Can I use other transfection reagents, such as PEI (polyethylenimine), in the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.
How scalable is the AAV-MAX transfection protocol?

The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.

Are the LV-MAX Enhancer and AAV-MAX Enhancer different?

Yes, these are different, and each is optimized for its intended use.

Will I see a drop in adeno-associated virus (AAV) titer if I don't use the AAV-MAX Enhancer with the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Enhancer is important for boosting AAV production. If the enhancer is not used, it will reduce system performance by 50% or more.
What is the optimal incubation time for the plasmid DNA/AAV-MAX Transfection Reagent/AAV-MAX Transfection Booster complex?

We recommend a complexation time of 20-30 mins. We recommend optimizing the complexation time for different sized plasmids.
Is it necessary to use the AAV-MAX Transfection Booster with the AAV-MAX Helper-Free AAV Production System? Will I see a drop in adeno-associated virus (AAV) titer if the booster is not used?

Yes, the AAV-MAX Transfection Booster facilitates transfection. If the booster is not used, it will reduce system performance.

Do I need to supplement the Viral Production Medium prior to use?

Yes, prior to using the Viral Production Medium, it should be supplemented with 4 mM GlutaMAX Supplement.
Can I use Viral Production Cells (Cat. No. A35347, A35827) with the AAV MAX Helper-Free AAV Production System?

The Viral Production Cells 2.0 are specifically designed to work with the other components of the AAV MAX Helper-Free AAV Production System to provide maximal adeno-associated virus (AAV) titers. Viral Production Cells could be used but we don't recommend doing this as it will reduce system performance.
What is the highest density that Viral Production Cells 2.0 can be cultured at?

The Viral Production Cells 2.0 achieve maximum cell densities of >1.2 x 10E7 cells/mL in shake flask cultures.
What is the passage stability of the Viral Production Cells 2.0?

We recommend using the Viral Production Cells 2.0 between passages 3 to 25 for adeno-associated virus (AAV) production.
How long do the Viral Production Cells 2.0 require to recover post-thaw before transfection?

We recommend allowing freshly thawed Viral Production Cells 2.0 to recover in culture for at least three passages prior to transfection.
Are the Viral Production Cells 2.0 a clonal cell line?

Yes, the Viral Production Cells 2.0 cells are a clonal cell line derived from single cell cloning of 293-F cells.

For Research Use Only. Not for use in diagnostic procedures.