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Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.
Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.
Commonly used as a second step for detection of biotinylated antibodies by ELISA and western blot. Avidin binds to biotin with high affinity. [Note: Sodium azide is an inhibitor of horseradish peroxidase enzymatic activity and should not be added to the AV-HRP diluent.]