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Near-infrared fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA.
The near IR fluorescent, lipophilic carbocyanine DiOC18(7) ('DiR') is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes.
When the lipophlilic probe laurdan is incorporated into membranes, its fluorescence spectra is sensitive to the physical state of the surrounding phospholipids.