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Invitrogen™ Ethidium Homodimer-1 (EthD-1)

Catalog No. E1169
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E1169 1 mg
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Catalog No. E1169 Supplier Invitrogen™ Supplier No. E1169
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Description

The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA.

The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (Excitation/emission maxima ∼528/617 nm).

We recommend using high-quality anhydrous DMSO for a stock concentration of 1 to 2 mM in DMSO/H2O 1:4 (v/v).

Order Info

Shipping Condition: Room Temperature

Specifications

Color Orange
Content And Storage Store in freezer (-5°C to -30°C) and protect from light.
Excitation Wavelength Range 528 nm
Dye Type Cell-Impermeant
For Use With (Application) Cell staining assays
For Use With (Equipment) Fluorescence Microscope
Quantity 1 mg
Detection Method Fluorescence
Description Ethidium Homodimer-1 (EthD-1)
Emission 617 nm
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Viability Indicator
SubCellular Localization Nucleic Acids, Nucleus
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Frequently Asked Questions (FAQs)

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

For Research Use Only. Not for use in diagnostic procedures.