Test
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.
Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Température de réaction optimale | 37°C |
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Enzymes | BamH I |
Catégorie de recherche | Traditional Cloning |
Sensibilité à la méthylation | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Type de produit | Restriction Enzyme |
Gamme de produits | FastDigest™ |
En surplomb | 5' protruding ends |
Enzymes de restriction de type IIS | No |
Tampon compatible | 10X FastDigest Buffer/FastDigest Green Buffer |
Inactivation Temperature | 80°C |
Sensible à l’inactivation thermique | Yes |
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).
The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Température de réaction optimale | 37°C |
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Enzymes | EcoR I |
Catégorie de recherche | Traditional Cloning |
Sensibilité à la méthylation | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Type de produit | Restriction Enzyme |
Gamme de produits | FastDigest™ |
En surplomb | 5' protruding ends |
Enzymes de restriction de type IIS | No |
Tampon compatible | 10X FastDigest Buffer/FastDigest Green Buffer |
Inactivation Temperature | 80°C |
Sensible à l’inactivation thermique | Yes |
Tampon de stockage | 10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol |
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Température de l’incubateur | 37°C |
Composants | 25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride |
Type de produit | EcoRI |
Site de coupe | G.AATTC |
pH | 7.4 |
Forme | Liquid |
Contenu et stockage | Keep container tightly closed |
À utiliser avec (application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI |
Tampon de stockage | 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol |
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Température de l’incubateur | 30°C |
Composants | 10X OPTIZYME™ Buffer 4 |
Type de produit | SmaI |
Site de coupe | CCC.GGG |
pH | 7.4 |
Concentration | 10 U/μL |
Forme | Liquid |
À utiliser avec (application) | >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI |
Tampon de stockage | 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol |
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Température de l’incubateur | 37°C |
Composants | >50% Water, 25 to 50% Glycerin |
Type de produit | ClaI (Bsu15I) |
Site de coupe | AT.CGAT |
pH | 7.4 |
Concentration | 10 U/μL |
Forme | Liquid |
Contenu et stockage | Keep container tightly closed |