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Protein Labeling Reagents

Various reagents designed to selectively label target proteins. Products include classic dyes, radioisotope labels, biotinylation reagents, fluorescent labels, molecular probes, assays, and labeling kits.
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1

Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Reagent, No-Weigh™ Format

Chemically tag purified proteins at primary amines to covalently crosslink and transfer biotinylation to protein interaction binding partners.

2

Invitrogen™ CPM (7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin)  DFS Item

The thiol-reactive coumarin, CPM is very weakly fluorescent until reacted with thiols producing a conjugate with excitation/emission maxima of ∼384/470 nm.

3

Thermo Scientific™ EZ-Link™ Maleimide-PEG11-Biotin

Biotinylate reduced cysteines with this long, maleimide-activated, sulfhydryl-reactive biotinylation reagent with an 11-unit PEG spacer arm to enhance solubility and reach.

4

Thermo Scientific™ Pierce™ Dye Removal Columns

Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.

5

Invitrogen™ Alexa Fluor™ 532 C5 Maleimide  DFS Item

Alexa Fluor™ 532 C5 Maleimide is a bright, yellow fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line.

6

Invitrogen™ Click-IT™ Palmitic Acid, Azide (15-Azidopentadecanoic Acid)  DFS Item
GREENER_CHOICE

Uses a simple and robust two-step labeling and detection technique

8

Invitrogen™ TAMRA, SE; 5-(and-6)-Carboxytetramethylrhodamine, Succinimidyl Ester (5(6)-TAMRA, SE), mixed isomers  DFS Item

TAMRA, SE; 5-(and-6)-Carboxytetramethylrhodamine, Succinimidyl Ester (5(6)-TAMRA, SE), mixed isomers

9

Thermo Scientific™ Alexa Fluor™ 420 NHS Ester (Succinimidyl Ester)
GREENER_CHOICE

Alexa Fluor 420 NHS Ester is an amine-reactive dye optimized for multiplex IHC and spatial biology. A long Stokes shift and 420/500 ex/em make it a good match for the CFP filter.

10

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Cell Permeability Cell-Impermeant
Product Type Sulfo-NHS-LC-Biotin
Spacer Mid-length
Label or Dye Biotin
Solubility DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water
Chemical Reactivity Amine
Product Line EZ-Link
11

Invitrogen™ Dansyl Ethylenediamine, 5-Dimethylaminonaphthalene-1-(N-(2-Aminoethyl))sulfonamide  DFS Item

5-dimethylaminonaphthalene-1-(N-(2-aminoethyl))sulfonamide (dansyl ethylenediamine)

14

Thermo Scientific™ DyLight™ 730-B1 NHS Ester

Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium- and cyanine-based, infrared-emitting (633 or 700 laser) NHS ester dyes.

15

Thermo Scientific™ EZ-Link™ Amine-PEG2-Biotin

Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with this short (20.4A) biotinylation reagent containing a 2-unit polyethylene glycol spacer.