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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

Corning™ Accutase™ Cell Detachment Solution, Liquid

Efficient, with gentle detachment of adherent cells within minutes

EDGE
Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
2

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

EDGE
3

Cytiva HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

EDGE
Content And Storage Store frozen
Sterilization Method 0.1 μm Sterile-filtered
Form Liquid
Shelf Life 24 Months
Source Porcine pancreas
4

Corning™ 10% SDS (Sodium Dodecyl Sulfate)

Powerful protein denaturant produced under highest industry standards

7

MilliporeSigma™ Calbiochem™ Trypsin Porcine MS Grade

A chemically modified trypsin that is resistant to autolysis and degradation

8

Gibco™ TrypLE™ Select Enzyme (10X), no phenol red
GREENER_CHOICE

TrypLE™ Select Enzyme (10X), no phenol red

Chelators EDTA
Purity or Quality Grade Cell Culture Grade
Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 10 X
Osmolality 270 - 320 mOsm/kg
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
pH 6.9 to 7.4
Shelf Life 24 Months
Product Line Gibco
10

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

11

MilliporeSigma™ Enzyme Free Cell Dissociation Solution Hank's Based (1x)

Hank's based formulation of chelating agents and agents to stabilize their activity on cells

Content And Storage 4°C
Formulation Chelating and stabilizing agents
12

MilliporeSigma™ Xeno-Free 0.05% Trypsin/0.02% EDTA Kit

Useful for routine detachment of cells from standard tissue plasticware and adhesion coated plasticware under animal-free conditions..

14

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Content And Storage -20°C
Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Calcium,No Magnesium,No Sodium Bicarbonate
pH 7 to 7.6
Sterility Sterile
15

Gibco™ TrypLE™ Express Enzyme (1X), phenol red  DFS Item
GREENER_CHOICE

Animal origin-free, recombinant enzyme

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco