Buffer and diluent solutions to prepare, dilute, and deliver protein and nucleic acid samples to electrophoresis gels for separation and analysis; includes solutions to stabilize, control the concentration of, denature, and reduce sample proteins.
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Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Quickly and easily remove high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis.
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.