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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

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1

Invitrogen™ SARS-CoV-2 Neutralizing Ab ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Content And Storage 2°C to 8°C
Host Species Human
Conjugate HRP
Interassay CV <10%
Intraassay CV <10
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Protein SARS-CoV-2 Spike Protein RBD
Test Time 40 mins
Regulatory Status RUO
Target SARS-CoV-2 Spike Protein RBD
Kit Contents Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
2

Invitrogen™ Mouse VCAM-1 ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Host Species Mouse
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Detectable Analytes VCAM-1
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Assay Sensitivity 1.5 pg/mL
Research Discipline Development
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Detection Method Colorimetric
Assay Solid-phase sandwich ELISA
Target VCAM-1 (CD106)
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6 Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 bet
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 1.5 pg/mL to 500 pg/mL
Interassay CV <12%
Gene Symbol CD106,Vcam-1
Storage Requirements 2°C to 8°C
Assay Range 2.05 to 500 pg/mL
Accession Number P29533
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 22329
Sample Type Plasma,Serum,Supernatant
Synonym CD106, Vcam-1
3

Invitrogen™ Porcine IL-6 ELISA Kit  DFS Item

ELISA

Protein Family Cytokines & Receptors
Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol IL6
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 45 pg/mL
Storage Requirements 2°C to 8°C
Research Discipline Immunology
Assay Range 40.96 to 10,000 pg/mL
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Detection Method Colorimetric
Assay Solid-phase sandwich ELISA
Accession Number P26893
Regulatory Status RUO
Target IL-6
Specificity The sandwich ELISA antibody pair detects Porcine IL-6.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 399500
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
4

Invitrogen™ Human TNFR1 (Soluble-60 kDa) ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Interassay CV 8.6%
Intraassay CV 1.9
Gene Symbol CD120a,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR1,TNFR55,TNFR60,p55,p55-R,p60
Total Assay Time 2 hrs 10 mins
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Urine 10 μL
Assay Sensitivity 0.05 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.08 to 5.0 ng/mL
For Use With (Application) ELISA
Test Time 1 hrs 20 mins
Accession Number P19438
Regulatory Status RUO
Target TNFR1
Kit Contents Pre-coated 96 well plates,Standard,Sample Diluent,Assay Buffer concentrate,Detection Antibody Conjugate: anti-rabbit-HRP,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 7132
Sample Type Plasma,Serum,Supernatant,Urine
Label or Dye HRP
Synonym Tumor necrosis factor receptor 1, TNF-R1, CD120a
5

Invitrogen™ Human IL-10RA (CD210a) ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 2.5 ng/mL
Research Discipline Signal Transduction
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target IL10RA
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 2.5 ng/mL to 500 ng/mL
Interassay CV <12%
Gene Symbol CD210,CD210a,CDW210A,HIL-10 R,IL-10R1,IL10R
Storage Requirements 2°C to 8°C
Assay Range 2.05 to 500 ng/mL
Accession Number Q13651
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 3587
Sample Type Plasma,Serum,Supernatant
Synonym CD210, CD210a, CDW210A, HIL-10 R, IL-10R1, IL10R
6

Invitrogen™ Human NGFR ELISA Kit  DFS Item

ELISA

Protein Family Growth Factors & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 80 pg/mL
Research Discipline Apoptosis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target NGFR (CD271)
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 80 pg/mL to 20 ng/mL
Interassay CV <12%
Gene Symbol CD271,Gp80-LNGFR,TNFRSF16,p75(NTR),p75NTR
Storage Requirements 2°C to 8°C
Assay Range 0.082 to 20 ng/mL
Accession Number P08138
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 4804
Sample Type Plasma,Serum,Supernatant
Synonym CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR
7

Invitrogen™ Human TRAIL R3/ TNFRSF10C ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Assay Sensitivity 15 pg/mL
Research Discipline Apoptosis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target TRAIL-R3 (CD263)
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 15 pg/mL to 4000 pg/mL
Interassay CV <12%
Gene Symbol CD263,DCR1,DCR1-TNFR,LIT,TRAIL-R3,TRAILR3,TRID
Storage Requirements 2°C to 8°C
Assay Range 16.38 to 4000 pg/mL
Accession Number O14798
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 8794
Sample Type Plasma,Serum,Supernatant
Synonym UNQ321/PRO366, CD263, DCR1, DCR1-TNFR, LIT, TRAIL-R3, TRAILR3, TRID
8

Invitrogen™ Human Thyroid Stimulating Hormone ELISA Kit  DFS Item

ELISA

Protein Family Hormones & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 4 pg/mL
Research Discipline Endocrinology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Thyroid Stimulating Hormone
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 4 pg/mL to 2000 pg/mL
Interassay CV <12%
Gene Symbol CG-ALPHA,FSHA,GPHA1,GPHa,HCG,LHA,TSHA
Storage Requirements 2°C to 8°C
Assay Range 2.744 to 2000 pg/mL
Accession Number P01215
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 1081, 7252
Sample Type Plasma,Serum,Supernatant
Synonym RP11-156F23.1, CG-ALPHA, FSHA, GPHA1, GPHa, HCG, LHA, TSHA
9

Invitrogen™ Mouse MIG/CXCL9 ELISA Kit  DFS Item

ELISA

Protein Family Chemokine & Receptors
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Assay Sensitivity 3 pg/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CXCL9 (MIG)
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 3 pg/mL to 2000 pg/mL
Interassay CV <12%
Gene Symbol BB139920,CMK,Mig,MuMIG,Scyb9,crg-10
Storage Requirements 2°C to 8°C
Assay Range 2.741 to 2000 pg/mL
Accession Number P18340
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 17329
Sample Type Plasma,Serum,Supernatant
Synonym BB139920, CMK, Mig, MuMIG, Scyb9, crg-10
10

Invitrogen™ Mouse Thrombopoietin (TPO/THPO) ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 21832
Interassay CV <12%
Intraassay CV <10
Assay Sensitivity 30 pg/mL
Assay Range 27.4 to 20,000 pg/mL
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
11

Invitrogen™ Porcine IL-4 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol Il4
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Quantity 96 Tests
Assay Sensitivity 1.5 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 1.5 to 400 pg/mL
Test Time 1 hrs 20 mins
Accession Number B cell growth factor 1; B
Regulatory Status RUO
Target IL-4
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 397225
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
12

Invitrogen™ Swine CSF2 (GM-CSF) ELISA Kit  DFS Item

ELISA

Protein Family Cytokines & Receptors
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 72 pg/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target GM-CSF
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-12, IFN-gamma, TGF beta 1, TNF alpha
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 assays
Calibration Range 72 pg/mL to 18,000 pg/mL
Interassay CV <12%
Gene Symbol GM-CSF
Storage Requirements 2°C to 8°C
Assay Range 73.73 to 18,000 pg/mL
Accession Number Q29118
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 397208
Sample Type Plasma,Serum,Supernatant
Synonym GM-CSF
13

Invitrogen™ Human IgG4 ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Intraassay CV 4.6
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 20 μL, Serum 20 μL
Assay Sensitivity 0.1 ng/mL
Assay Range 9.4 to 600 ng/mL
For Use With (Application) ELISA
Test Time 1 hrs 20 mins
Regulatory Status RUO
Target Human IgG4
Kit Contents Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3503
Sample Type Plasma,Serum
Synonym Immunoglobulin G4
14

Invitrogen™ Human I-TAC/CXCL11 ELISA Development Kit (ABTS), PeproTech®

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6373
Assay Sensitivity Lot-specific
Assay Range Lot-specific
15

Invitrogen™ Mouse IL-13 ELISA Development Kit (ABTS), PeproTech®

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 16163
Assay Sensitivity Lot-specific
Assay Range Lot-specific