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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (25)
Singleplex and Multiplex Bead Based Immunoassays (11)
ELISA Substrates and Reagents (1)

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1 – 15 of 36 results
2

Applied Biosystems™ cAMP-Screen Direct™ Cyclic AMP Immunoassay System

Ultrasensitive determination of cyclic AMP (cAMP) levels in cell lysates with a simplified protocol. Thermo Scientific cAMP-Screen Direct 96-well Cyclic AMP Immunoassay System is a competitive chemiluminescent immunoassay formatted with maximum flexibilit

Protein Family GPCR (G-Protein Coupled Receptors)
Content And Storage 1 kit, store at 2°C to 8 °C
For Use With (Equipment) Microplate Reader
Gene ID (Entrez) Non-Gene
Sample Type Cell Culture
Label or Dye AP
Gene Symbol Non-Gene
Sample Volume 60 μL
Assay Sensitivity 60 femtomoles cAMP
Target Kit Named Cyclic AMP
Product Line cAMP-Screen Direct
Detection Method Chemiluminescence
3

R&D Systems™ Mouse GM-CSF Quantikine ELISA Kit

Mouse GM-CSF Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Target Species Mouse
Conjugate HRP
Gene ID (Entrez) 12981
Sample Type Cell Culture Supernatant,Serum
Sample Volume Cell Culture Supernates (50uL),Serum (50uL)
Synonym colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF2, CSF-2, GMCSF, GM-CSF, GMCSFgranulocyte-macrophage colony-stimulating factor, granulocyte-macrophage colony stimulating factor, MGC131935, MGC138897, Molgramostim, molgramostin, sargramostim
Assay Sensitivity 5.8pg/mL
Assay Range 7.80 - 500pg/mL
For Use With (Application) ELISA
Cross Reactivity <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules
4

Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

6

Invitrogen™ Human FcGR2BC (CD32 B/C) ELISA Kit

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 2.5 μL, Serum 2.5 μL, Supernatant 100 μL
Assay Sensitivity 85 pg/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CD32
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 85 pg/mL to 5000 pg/mL
Interassay CV <12%
Gene Symbol CD32,CD32B,FCG2,FCGR2,IGFR2,CD32,FCG2,CD32C,CDW32,IGFR2,FCRIIC
Storage Requirements 2°C to 8°C
Assay Range 78.13 to 5000 pg/mL
Accession Number P31994,P31995
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 2212, 2213, 9103
Sample Type Plasma,Serum,Supernatant
Synonym RP11-474I16.2, CD32, CD32B, FCG2, FCGR2, IGFR2
7

Invitrogen™ Rat Fas Ligand ELISA Kit

ELISA

Protein Family Other Proteins
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 90 pg/mL
Research Discipline Apoptosis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Fas Ligand (CD178)
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 assays
Interassay CV <12%
Gene Symbol Faslg
Storage Requirements 2°C to 8°C
Assay Range 82.30 to 60,000 pg/mL
Accession Number P36940
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 25385
Sample Type Plasma,Serum,Supernatant
Synonym Apt1Lg1, CD95-L, Fasl, Tnfsf6
8

Invitrogen™ Apolipoprotein E4 Human ELISA Kit

Sandwich ELISA Kit

Protein Family GCPR
Target Species Human
Conjugate Biotin
Interassay CV <12%
Intraassay CV <10%
Gene Symbol AD2,APO-E,ApoE4,LDLCQ5,LPG
Total Assay Time 4 hr. 45 min.
Assay Sensitivity 0.4 ng/mL
Storage Requirements 2°C to 8°C
Research Discipline Cardiovascular Research
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr. 20 min.
Assay Solid-phase sandwich ELISA
Accession Number P02649
Regulatory Status RUO
Target Human
Specificity This ELISA pair antibody detects human ApoE4. Other species not determined.
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 348
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym AD2, APO-E, LDLCQ5, LPG
9

Invitrogen™ Human ErbB2 (HER2) ELISA Kit

ELISA

Protein Family Proteases, Peptidases & Inhibitors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 8 pg/mL
Research Discipline Angiogenesis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Her-2 (ErbB2)
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 8 pg/mL to 2000 pg/mL
Interassay CV <12%
Gene Symbol ERBB2
Storage Requirements 2°C to 8°C
Assay Range 8.19 to 2000 pg/mL
Accession Number P04626
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 2064
Sample Type Plasma,Serum,Supernatant
Synonym CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1
10

Invitrogen™ Human TGFBR2 ELISA Kit

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
Assay Sensitivity 15 pg/mL
Research Discipline Apoptosis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target TGFBR2
Specificity This ELISA antibody pair detects human TGF-beta R2. Other species not determined yet.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 1.5 pg/mL to 500 pg/mL
Interassay CV <12%
Gene Symbol AAT3,FAA3,LDS1B,LDS2,LDS2B,MFS2,RIIC,TAAD2,TGFR-2,TGFbeta-RII
Storage Requirements 2°C to 8°C
Assay Range 2.05 to 500 pg/mL
Accession Number P37173
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 7048
Sample Type Plasma,Serum,Supernatant
Synonym AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, TGFbeta-RII
11

Invitrogen™ Human Ubiquitin ELISA Kit

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 30 pg/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Ubiquitin
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 A, IL-1 b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-g, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 A, MIP-1 b, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-b, TIMP-1, TIMP-2, TNF-a, TNF-b, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Interassay CV <12%
Gene Symbol CEP80,HEL112,S27A,UBA80,UBC,UBCEP1,UBCEP80
Storage Requirements 2°C to 8°C
Assay Range 27.43 to 20,000 pg/mL
Accession Number P62979
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 51065, 6233, 7311, 7314, 7316
Sample Type Plasma,Serum,Supernatant
Synonym CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80
12

Invitrogen™ Human Serpin A1 ELISA Kit

ELISA

Protein Family Proteases, Peptidases & Inhibitors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Assay Sensitivity 2 ng/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target alpha-1 Antitrypsin
Specificity This ELISA pair antibody detects human Serpin A1. Other species not determined.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 2 ng/mL to 500 ng/mL
Interassay CV <12%
Gene Symbol A1A,A1AT,AAT,PI,PI1,PRO2275,alpha1AT
Storage Requirements 2°C to 8°C
Assay Range 2.05 to 500 ng/mL
Accession Number P01009
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 5265
Sample Type Plasma,Serum,Supernatant
Synonym PRO0684, A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT
13

Invitrogen™ Human Cardiac Troponin I (TNNI3) ELISA Kit

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 100 pg/mL
Research Discipline Cardiovascular Research
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Cardiac Troponin I
Specificity This ELISA pair antibody detects human cTnI. Other species not determined.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 100 pg/mL to 8000 pg/mL
Interassay CV <12%
Gene Symbol CMD1FF,CMD2A,CMH7,RCM1,TNNC1,cTnI
Storage Requirements 2°C to 8°C
Assay Range 0.38 to 625 pg/ml
Accession Number P19429
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 7137
Sample Type Plasma,Serum,Supernatant
Synonym CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI
14

Invitrogen™ Human Luteinizing Hormone ELISA Kit

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 140 pg/mL
Research Discipline Signal Transduction
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Luteinizing Hormone
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 140 pg/mL to 10,000 pg/mL
Interassay CV <12%
Gene Symbol CGB4,HH23,LSH-B,LSH-beta
Storage Requirements 2°C to 8°C
Assay Range 156.3 to 10,000 pg/mL
Accession Number P01229
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 1081, 3972
Sample Type Plasma,Serum,Supernatant
Synonym RP11-156F23.1, CG-ALPHA, FSHA, GPHA1, GPHa, HCG, LHA, TSHA
15

Invitrogen™ Human Amyloid beta 42 ELISA Kit, Ultrasensitive

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 351
Interassay CV 7.5%
Intraassay CV 8.6
Assay Sensitivity <1 pg/mL
Assay Range 1.56 to 100 pg/mL