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Thermo Scientific™ Pierce™ Human IFN alpha ELISA Kits (for Supernatant)


Measure levels of human interferon alpha (IFNa) in human cell and tissue culture media supernatant samples using 96-well plates.

Manufacturer: thermo scientific™  411002

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Catalog No. PI411002

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Description & Specifications

Specifications

Assay Range High Sensitivity: 12.5 to 500pg/mL; Extended Range: 156 to 5000pg/mL
Description Human IFN alpha ELISA Kit (Supernatant)
Format Colorimetric ELISA (TMB substrate) in clear 96-well plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents Antibody Concentrate, 1 vial
Precision Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
Measurement Absorbance at 450nm
Quantity 5 plate kit
Sensitivity Undetermined
Sample Type Cell culture supernatant, tissue culture media
Sample Size 100µL
Specificity No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß or IFN-g; bovine IFN-t
Sufficient For 5 x 96 microplate wells
Total Assay Time 3.25 hr.
Target Human interferon alpha (human IFN-a, IFN-alpha)

Assay IFN-a (human interferon alpha) levels in human cell and tissue culture media (supernatants) with these Thermo Scientific™ Human IFN alpha ELISA Kits (for Supernatant).

These human IFN alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 100μL per well. These IFN-α kits are validated for use with human cell and tissue culture media and are optimized for both high-sensitivity and extended assay ranges. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Human interferon alpha (human IFN-α, IFN-alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well plate

  • Measurement: Absorbance at 450nm

  • Assay Range: 12.5 to 500pg/mL (high sensitivity); 156 to 5000pg/mL (extended range)

  • Sensitivity: Undetermined

  • Precision: Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%

  • Sample Types: Cell culture supernatant, tissue culture media

  • Total Assay Time: 3.25 hours

  • Sample Size: 100μL

  • Specificity: No significant cross-reaction with human IFN-β, IFN-γ or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; bovine IFN-τ

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.