ELISA Reagents

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Thermo Scientific™ Pierce™ TMB Substrate Kit

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Invitrogen™ NuPAGE™ Transfer Buffer (20X) DFS Item

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

Thermo Scientific™ 1-Step™ Turbo TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ Bolt™ Antioxidant DFS Item

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Thermo Scientific™ SuperBlock™ (PBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X) DFS Item

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NativePAGE™ Running Buffer (20X) DFS Item

Used to make the NativePAGE Cathode and Anode Running Buffers

Goat Anti-Mouse IgG (H+L), HRP, Secondary Antibody, Promega DFS Item

Ideal for use in protein detection in Western Blots and ELISAs.

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

Thermo Scientific™ 6X DNA Loading Dye Promotion

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Goat Anti-Rabbit IgG (H+L), HRP, Secondary Antibody, Promega DFS Item

Ideal for use in protein detection in Western Blots and ELISAs.

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Invitrogen™ eBioscience™ IC Fixation Buffer

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Thermo Scientific™ SuperBlock™ T20 (TBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007 DFS Item

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer

Thermo Scientific™ PNPP Substrate Tablets

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

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