ELISA Reagents

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ Pierce™ TMB Substrate Kit

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ NuPAGE™ Transfer Buffer (20X) DFS Item

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X) DFS Item

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Thermo Scientific™ SuperBlock™ (PBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Invitrogen™ Dynabeads™ MyOne™ Streptavidin C1 DFS Item

Exceptional standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets

Thermo Scientific™ HRP-Conjugated Streptavidin

Streptavidin from Streptomyces avidinii conjugated to highly purified horseradish peroxidase (HRP)

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

Invitrogen™ NuPAGE™ Antioxidant DFS Item

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ Bolt™ Antioxidant DFS Item

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X) DFS Item

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X) DFS Item

Made with high-purity reagents and are strictly quality controlled

Thermo Scientific™ SuperBlock™ T20 (TBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Invitrogen™ eBioscience™ IC Fixation Buffer

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Goat Anti-Mouse IgG (H+L), HRP, Secondary Antibody, Promega DFS Item

Ideal for use in protein detection in Western Blots and ELISAs.

Invitrogen™ eBioscience™ Fixation/Permeabilization Diluent

Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X) DFS Item

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

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