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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1 – 15 of 62 results
1

Gibco™ Trypsin (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Shipping Condition Wet Ice
Chelators No EDTA
Content And Storage Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality 280 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
2

Gibco™ Trypsin (2.5%), no phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

3

Gibco™ Cell Dissociation Buffer, enzyme-free, PBS  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

4

Gibco™ Soybean Trypsin Inhibitor, powder  DFS Item

Trypsin inhibited at a molar ratio of 1:1 and chymotrypsin and plasmin to lesser extent

5

Gibco™ Collagenase, Type I, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Product Type Collagenase
Form Lyophilized
Shelf Life 24 Months
Sterility Non-sterile
6

Gibco™ Collagenase, Type II, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen

7

Gibco™ Dispase II, powder  DFS Item

Amino-endo peptidase that hydrolyzes N-terminal peptide bonds of non-polar amino acid residues

8

MilliporeSigma™ Xeno-Free 0.05% Trypsin/0.02% EDTA Kit

Useful for routine detachment of cells from standard tissue plasticware and adhesion coated plasticware under animal-free conditions..

10

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red  DFS Item
GREENER_CHOICE

Unlike porcine trypsin, free of animal-derived components

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
11

MilliporeSigma™ Chemicon™ Accumax™ Cell Detachment Solution

A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

12

MilliporeSigma™ Trypsin 0.25%

The Trypsin 0.25%, In Hank's Balanced Salt Solution, without Ca 2+ and Mg 2+ is available in a 100mL format.

13

Gibco™ Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS).

14

Gibco™ TrypLE™ Select Enzyme (10X), no phenol red  DFS Item
GREENER_CHOICE

TrypLE™ Select Enzyme (10X), no phenol red

Chelators EDTA
Purity or Quality Grade Cell Culture Grade
Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 10 X
Osmolality 270 - 320 mOsm/kg
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
pH 6.9 to 7.4
Shelf Life 24 Months
Product Line Gibco