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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

EDGE
2

Corning™ Accutase™ Cell Detachment Solution, Liquid

Efficient, with gentle detachment of adherent cells within minutes

EDGE
Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
3

MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x)

Used to dissociate primary cells, tissues, and tumors with an increased plating efficiency. MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x) is PBS based formulation of chelating agents that contain agents to stabilize their activity on cells.

Content And Storage Store at room temperature or at 4°C.
Product Type Cell Culture Dissociation Reagent
Form Liquid
Formulation Chelating and stabilizing agents
Shelf Life 2 Years
Concentration 1 X
For Use With (Application) Cell Dissociation
Sterility Sterile
4

MilliporeSigma™ Enzyme Free Cell Dissociation Solution Hank's Based (1x)

Hank's based formulation of chelating agents and agents to stabilize their activity on cells

Content And Storage 4°C
Formulation Chelating and stabilizing agents
5

Corning™ 0.25% Trypsin in HBSS w/o Calcium and Magnesium

Enzymatic dissociation reagents tested for porcine parvovirus

6

Cytiva HyrTrp Cell Dissociation Reagent

Recombinant and animal-derived component-free alternative to traditional porcine trypsin

Content And Storage -20 °C
For Use With Attachment-dependent cell types MRC-5, Vero, CHO K-1 cell lines grown with or without serum
Product Type Cell Culture Dissociation Reagent
Shelf Life 12 months from manufacturing date when stored at -20°C
7

MilliporeSigma™ Chemicon™ Accumax™ Cell Detachment Solution

A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

8

MilliporeSigma™ Trypsin 0.25%

The Trypsin 0.25%, In Hank's Balanced Salt Solution, without Ca 2+ and Mg 2+ is available in a 100mL format.

9

Gibco™ Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS).

11

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

12

Gibco™ Trypsin-EDTA (0.5%), no phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

14

Gibco™ Collagenase, Type I, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Product Type Collagenase
Form Lyophilized
Shelf Life 24 Months
Sterility Non-sterile
15

MilliporeSigma™ Xeno-Free 0.05% Trypsin/0.02% EDTA Kit

Useful for routine detachment of cells from standard tissue plasticware and adhesion coated plasticware under animal-free conditions..