Oligonucleotides with a wide variety of applications in microarrays, gel shift assays, artificial gene synthesis, PCR, DNA sequencing, molecular cloning, and other custom procedures. Available in multiple primer lengths; may be pre-designed or random.
Thermo Scientific™ Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
Anchored Oligo(dT)20 Primer is a primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (dG, dA, or dC) and then by dN (dA, dT, dG, or dC).
Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C, Oligo(dT)20 Primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA.
Use this set of oligonucleotides for template switching cDNA synthesis and preamplification. Template Switching Oligonucleotide and Capturing Oligo (dT) are used to incorporate adaptor sequences to full-length cDNA. Preamplification primer enables PCR amplification of obtained cDNA.
Random Decamers are the same primers currently included in the RETROscript™ Kit (Cat. No. AM1710). They are provided at a stock concentration of 50 μM, and are functionally tested using the RETROscript™ Kit.