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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
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1
Promotions Available

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
2
Promotions Available

Applied Biosystems™ SYBR™ Green Universal Master Mix  DFS Item

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Label or Dye SYBR Green
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line SYBR
Detection Method SYBR
3

Invitrogen™ dNTP Mix (10 mM ea)  DFS Item

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

Shipping Condition Approved for shipment on Wet or Dry Ice
Form Liquid
Label or Dye Unlabeled
Concentration 10 mM
For Use With (Application) Standard PCR,RT-PCR,Real Time PCR (qPCR)
4
Promotions Available

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR  DFS Item

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 8 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Product Line Applied Biosystems
5

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Reaction Speed Standard
Hot Start No
Overhang 3'-A
Concentration 5 U/μL
6

Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay  DFS Item

The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.

Shipping Condition Dry Ice
Format Kit
Label or Dye VIC
Species Not Species-Specific
Product Line VetMAX, Xeno
7

Thermo Scientific™ dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 10 mM
8

Applied Biosystems™ SYBR™ Select Master Mix  DFS Item

Cost-effective solution for real-time PCR applications.

GC-Rich PCR Performance High
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq DNA Polymerase
Format Vial
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Label or Dye SYBR GreenER
Hot Start Built-In Hot Start
Sufficient For 100 Reactions of 20 μL
Product Line SYBR
9

Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer

The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.

10

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

11

Invitrogen™ Platinum™ Taq DNA Polymerase  DFS Item

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Starting Material DNA
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
12

Invitrogen™ MultiScribe™ Reverse Transcriptase  DFS Item

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.

13

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit  DFS Item

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 120 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Quantity 1 Ea.
Technique Reverse Transcription
Concentration 50 U/μL
Starting Material RNA
14
Promotions Available

Invitrogen™ PureLink™ PCR Micro Kit  DFS Item

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

Shipping Condition Room Temperature
Separation Range 100 bp to 12 kb
Content And Storage Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Starting Material Volume 125 μL
Isolation Technology Silica Spin Column
Elution Volume Max 20 μL
For Use With (Application) Cloning,Standard PCR
High-throughput Compatibility Not High-throughput Compatible (Manual)
Target DNA from Enzymatic Reactions (e.g. PCR)
Format Spin Column
Product Type PCR Micro Kit
Run Time 10 min.
Elution Volume Min 5 μL
Product Line PureLink
15

Applied Biosystems™ MicroSEQ™ D2 rDNA Fungal PCR Kit
GREENER_CHOICE

This kit is the PCR component of the MicroSEQ™ Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi.