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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (4,043)
Singleplex and Multiplex Bead Based Immunoassays (527)
ELISA Substrates and Reagents (78)

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1

Invitrogen™ SARS-CoV-2 Neutralizing Ab ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Content And Storage 2°C to 8°C
Host Species Human
Conjugate HRP
Interassay CV <10%
Intraassay CV <10
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Protein SARS-CoV-2 Spike Protein RBD
Test Time 40 mins
Regulatory Status RUO
Target SARS-CoV-2 Spike Protein RBD
Kit Contents Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
2

Invitrogen™ Human MIP-1a ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6348
Interassay CV 7.1%
Intraassay CV 6.5
Assay Sensitivity <2 pg/mL
Assay Range 15.6 to 1000 pg/mL
For Use With (Application) Colorimetric
3

Invitrogen™ Mouse IFN gamma ELISA Kit  DFS Item

ELISA

Target Species Mouse
Conjugate HRP
Interassay CV 6.5%
Intraassay CV 3.2
Incubation Time 4 hr.
Gene Symbol IFN-g,Ifg
Total Assay Time 4 hrs
Sample Volume Plasma 25 μL, Serum 25 μL, Supernatant 25 μL
Assay Sensitivity <2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 47 to 300 pg/mL
For Use With (Application) Colorimetric Detection
Test Time 1 hrs 20 mins
Accession Number P01580
Regulatory Status RUO
Target IFN gamma
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 15978
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
4

Invitrogen™ Human CRP ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1401
Interassay CV 9.7%
Intraassay CV 7
Assay Sensitivity <10 pg/mL
Assay Range 18.75 to 1200 pg/mL
For Use With (Application) Colorimetric Detection
5

Invitrogen™ Human IL-6 ELISA Kit  DFS Item

ELISA

Target Species Human
Conjugate HRP
Interassay CV 7.9%
Intraassay CV 6.2
Gene Symbol BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
Total Assay Time 3 hrs
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity <2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 7.8 to 2500 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hrs 20 mins
Accession Number P05231
Regulatory Status RUO
Target IL-6
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision <2 pg/mL
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3569
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
6

Invitrogen™ Mouse IL-17B ELISA Kit  DFS Item

ELISA

Protein Family Cytokines & Receptors
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 12 pg/mL
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target IL17B
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-5, M-CSF, MIG, MIP- 1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Interassay CV <12%
Gene Symbol 1110006O16Rik,1700006N07Rik,Zcyto7
Storage Requirements 2°C to 8°C
Assay Range 16.46 to 12,000 pg/mL
Accession Number Q9QXT6
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 56069
Sample Type Plasma,Serum,Supernatant
Synonym 1110006O16Rik, 1700006N07Rik, Zcyto7
7

Invitrogen™ Mouse IL-1RA ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 20 μL, Serum 20 μL, Supernatant 50 μL
Assay Sensitivity 2 pg/mL
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target IL1RA
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 2 pg/mL to 2000 pg/mL
Interassay CV <12%
Gene Symbol F630041P17Rik,IL-1 ra
Storage Requirements 2°C to 8°C
Assay Range 2.74 to 2000 pg/mL
Accession Number P25085
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 16181
Sample Type Plasma,Serum,Supernatant
Synonym RP23-218B17.2, F630041P17Rik, IL-1 ra
8

Invitrogen™ 20-Hydroxyecdysone ELISA Kit

ELISA

Conjugate HRP
Interassay CV 9%
Intraassay CV 10
Total Assay Time 2 hrs 30 mins
Sample Volume Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
Assay Sensitivity 197 pg/mL
Storage Requirements -20°C
Assay Range 102.4 to 25,000 pg/mL
Test Time 45 mins
Accession Number 20 E; C27H44O7; ecdysterone
Regulatory Status RUO
Target 20-Hydroxyecdysone
Kit Contents Anti-IgG coated 96 well plate,Standard,Assay Buffer Concentrate,Capture Antibody,HRP-conjugated Antigen,Wash Buffer Concentrate,TMB Substrate,Stop Solution,Plate Sealer
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye HRP
9

Invitrogen™ Human IgA ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Interassay CV 4.8%
Intraassay CV 3.9
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 10 μL, Serum 10 μL
Assay Sensitivity 0.03 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 1.6 to 100 ng/mL
For Use With (Application) ELISA
Test Time 1 hrs 20 mins
Regulatory Status RUO
Target Human IgA
Kit Contents Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3493, 3494
Sample Type Plasma,Serum
Label or Dye HRP
Synonym Immunoglobulin A
10

Invitrogen™ Mouse DAN (PARN) ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 35 pg/mL
Research Discipline Other Research
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target PARN
Specificity This ELISA pair antibody detects mouse DAN, no cross-reactivity with rhDAN, rhCOCO, rmBMP-4 or rmGremlin
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 35 pg/mL to 10,000 pg/mL
Interassay CV <12%
Gene Symbol 1200003I18Rik,DAN
Storage Requirements 2°C to 8°C
Assay Range 40.96 to 10,000 pg/mL
Accession Number Q8VDG3
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 74108
Sample Type Plasma,Serum,Supernatant
Synonym 1200003I18Rik, DAN
11

Invitrogen™ Mouse Renin 1 (REN1) ELISA Kit  DFS Item

ELISA

Protein Family Proteases, Peptidases & Inhibitors
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 6 pg/mL
Research Discipline Neurobiology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Renin
Specificity This ELISA antibody pair detects mouse Renin. Other species not determined yet
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 6 pg/mL to 50000 pg/mL
Interassay CV <12%
Gene Symbol D19352,Ren,Ren-1,Ren-Ac,Ren1d,Rn-1,Rnr,Ren1
Storage Requirements 2°C to 8°C
Assay Range 12.21 to 50,000 pg/mL
Accession Number P06281
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 19701
Sample Type Plasma,Serum,Supernatant
Synonym D19352, Ren, Ren-1, Ren-Ac, Ren1d, Rn-1, Rnr, Ren1
12

Invitrogen™ Mouse Resistin (RETN) ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Assay Sensitivity 5 pg/mL
Research Discipline Development
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Resistin
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 5 pg/mL to 1000 pg/mL
Interassay CV <12%
Gene Symbol ADSF,Fizz3,Rstn,Xcp4
Storage Requirements 2°C to 8°C
Assay Range 4.096 to 1000 pg/mL
Accession Number Q99P87
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 57264
Sample Type Plasma,Serum,Supernatant
Synonym ADSF, Fizz3, Rstn, Xcp4
13

Invitrogen™ Human STAT5 alpha/beta InstantOne™ ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6776, 6777
15

Invitrogen™ Human/Mouse TGF beta-1 Uncoated ELISA Kit with Plates

ELISA

Target Species Human,Mouse
Conjugate HRP
Gene Symbol Tgfb1
Total Assay Time 24 hr 30 min
Sample Volume Plasma 20 μL, Serum 20 μL, Supernatant 100 μL
Assay Sensitivity 15.6 pg/mL
Storage Requirements 2-8°C
Assay Range 15.6-1000 pg/mL
For Use With (Application) ELISA
Test Time 1 hr 45 min
Accession Number P01137, P04202
Regulatory Status RUO
Target TGF beta-1
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 21803, 7040
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
Synonym Transforming Growth Factor beta 1, TGF-b1