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  5. Invitrogen™ Human ENA-78/CXCL5 ELISA Kit

Invitrogen™ Human ENA-78/CXCL5 ELISA Kit

Catalog No. EHCXCL5
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96 Tests
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Catalog No. Quantity
EHCXCL5 96 Tests
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Catalog No. EHCXCL5 Supplier Invitrogen™ Supplier No. EHCXCL5

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Description

ELISA

The Human ENA-78/CXCL5 (ENA-78) ELISA quantitates Hu ENA-78 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ENA-78. Principle of the method The Human BTC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.

Specifications

Accession Number P42830
Assay Range 10-18000 pg/mL
Assay Sensitivity 10 pg/mL
Calibration Range 10 pg/mL to 18000 pg/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Chemokines & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -7, -8, -9, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias AMCF-II; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 5; chemokine (C-X-C motif) ligand 6; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); CXC; CXC chemokine LIX; CXC motif chemokine; C-X-C motif chemokine 5; C-X-C motif chemokine ligand 5; CXCL; CXCL5; Cxcl6; Cytokine LIX; ENA78; ENA-78; ENA-78(1-78); ENA-78(8-78); ENA-78(9-78); Epithelial-derived neutrophil-activating protein 78; GCP-2; GCP-2(1-78); GCP-2(9-78); granulocyte chemotactic protein-2; LIX; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; Scyb5; Scyb6; small inducible cytokine B subfamily, member 5; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); small inducible cytokine subfamily B, member 15; small-inducible cytokine B5AMCF-II; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 5; chemokine (C-X-C motif) ligand 6; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); CXC; CXC chemokine LIX; CXC motif chemokine; C-X-C motif chemokine 5; C-X-C motif chemokine ligand 5; CXCL; CXCL5; Cxcl6; Cytokine LIX; ENA78; ENA-78; ENA-78(1-78); ENA-78(8-78); ENA-78(9-78); Epithelial-derived neutrophil-activating protein 78; GCP-2; GCP-2(1-78); GCP-2(9-78); granulocyte chemotactic protein-2; LIX; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; Scyb5; Scyb6; small inducible cytokine B subfamily, member 5; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); small inducible cytokine subfamily B, member 15; small-inducible cytokine B5; ENA-78; SCYB5
Gene ID (Entrez) 6374
Gene Symbol CXCL5
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Inflammation
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym ENA-78, SCYB5
Target CXCL5
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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