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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (4,053)
Singleplex and Multiplex Bead Based Immunoassays (527)
ELISA Substrates and Reagents (78)

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1

Invitrogen™ SARS-CoV-2 Neutralizing Ab ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Content And Storage 2°C to 8°C
Host Species Human
Conjugate HRP
Interassay CV <10%
Intraassay CV <10
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Protein SARS-CoV-2 Spike Protein RBD
Test Time 40 mins
Regulatory Status RUO
Target SARS-CoV-2 Spike Protein RBD
Kit Contents Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
2

Invitrogen™ Human Insulin ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Protein Family Hormones & Receptors
Immunoassay Kit Format Sandwich ELISA Kit
Target Species Human
Conjugate HRP
Interassay CV 8.5%
Intraassay CV 5.4%
Gene Symbol INS
Total Assay Time 45 min
Assay Sensitivity 0.17 μIU/mL
Storage Requirements 2-8°C
Assay Range 5.1-250 μlU/mL
Test Time 1 hr 20 min
Accession Number P01308
Regulatory Status RUO
Target Insulin
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Controls,Standard,Standard Dilution Buffer,Incubation Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Controls
Gene Alias AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulinAA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin; IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10
Product Type ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene ID (Entrez) 3630
Sample Type Serum
Label or Dye Biotin
3

Invitrogen™ Human Prostaglandin E2 ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 11.7%
Intraassay CV 6
Assay Range 31.3 to 4000 pg/mL
For Use With (Application) Colorimetric Detection
5

Invitrogen™ Human Amyloid beta (Aggregated) ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 351
Interassay CV 7.4%
Intraassay CV 7.4
Assay Sensitivity <0.01 ng/mL
Assay Range 0.09 to 5.7 ng/mL
7

Invitrogen™ Human TGFb1/LAP Uncoated ELISA Kit with Plates

ELISA

Protein Aliases TGF-beta-1, LAP
Immunoassay Kit Format Uncoated ELISA Kit with Plates
Content And Storage Capture Antibody Concentrate
Detection Antibody Concentrate
Human LAP standard (lyophilized): 20 ng/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent B
pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution
96 Well Plate included with product Cat. Nos. ending in suffixes -22, -76, -86
Target Species Human
Conjugate HRP
Gene Symbol Tgfb1
Total Assay Time 24 hr 30 min
Assay Sensitivity 156.3 pg/mL
Storage Requirements 2-8°C
Assay Range 156.3-10000 pg/mL
Test Time 1 hr 45 min
Accession Number P01137
Regulatory Status RUO
Target TGF beta-1
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Gene Alias CED; cytokine; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGF b; TGF b 1; TGF beta; tgf beta 1; tgf beta1; TGF beta-1; TGF beta-1 protein; TGF β; TGF β 1; TGF β1; Tgfb; TGFB1; TGF-B1; Tgfb-1; TGFbeta; TGF-beta 1; TGF-beta 1 protein; TGFbeta1; TGF-beta1; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor beta-1 proprotein; transforming growth factor beta-1; transforming growth factor β1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1; transforming growth factor-beta I; transforming growth factor-beta-1; transforming growth factor-beta-1 precursor; unnamed protein productCED; cytokine; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGF b; TGF b 1; TGF beta; tgf beta 1; tgf beta1; TGF beta-1; TGF beta-1 protein; TGF β; TGF β 1; TGF β1; Tgfb; TGFB1; TGF-B1; Tgfb-1; TGFbeta; TGF-beta 1; TGF-beta 1 protein; TGFbeta1; TGF-beta1; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor beta-1 proprotein; transforming growth factor beta-1; transforming growth factor β1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1; transforming growth factor-beta I; transforming growth factor-beta-1; transforming growth factor-beta-1 precursor; unnamed protein product; CED; DPD1; LAP; TGFB; TGFbeta
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 7040
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
8

Invitrogen™ Human IgM ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Interassay CV 3.2%
Intraassay CV 5.1
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity 1 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 15.6 to 1,000 ng/mL
For Use With (Application) ELISA
Test Time 1 hrs 20 mins
Regulatory Status RUO
Target Human IgM
Kit Contents Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3507
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Immunoglobulin M
9

Invitrogen™ Human IL-4 ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Human
Conjugate FAM™
Incubation Time 1 hr.
Gene Symbol BCGF-1; BCGF1; BSF-1; BSF1; IL-4
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.01 pg/mL
Storage Requirements Frozen at -20°C
Assay Range 0.0128 to 5000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P05112
Regulatory Status RUO
Target IL-4
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 3565
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
10

Invitrogen™ Human TROP1/EpCAM ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Assay Sensitivity 45 pg/mL
Research Discipline Cell Cycle & Proliferation
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target EpCAM (CD326)
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Interassay CV <12%
Gene Symbol DIAR5,EGP-2,EGP314,EGP40,ESA,HNPCC8,KS1/4,KSA,M4S1,MIC18,MK-1,TACSTD1,TROP1
Storage Requirements 2°C to 8°C
Assay Range 49.15 to 12,000 pg/mL
Accession Number P16422
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 4072
Sample Type Plasma,Serum,Supernatant
Synonym DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1
11

Invitrogen™ Mouse IL-5 ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 16191
Interassay CV <12%
Intraassay CV <10
Assay Sensitivity 1 pg/mL
Assay Range 2.05 to 500 pg/mL
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
12

Invitrogen™ Human IL-21 Uncoated ELISA Kit with Plates

ELISA

Target Species Human
Conjugate HRP
Total Assay Time 24 hr 30 min
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 8 pg/mL
Assay Range 8-1000 pg/mL
For Use With (Application) ELISA
Test Time 1 hr 45 min
Regulatory Status RUO
Target IL-21
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 59067
Sample Type Plasma,Serum,Supernatant
Synonym Interleukin-21
13

Invitrogen™ Human IL-1 beta Uncoated ELISA Kit with Plates

ELISA

Target Species Human
Conjugate HRP
Gene Symbol IL-21
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 2 to 150 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Accession Number P01584
Regulatory Status RUO
Target IL-1 beta
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3553
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-1 beta
15

Thermo Scientific™ CaptureSelect™ IgG-CH1 Ligand Leakage ELISA
GREENER_CHOICE

Thermo Scientific™ CaptureSelect™ IgG-CH1 Ligand Leakage ELISA is designed to detect possible leached ligand of antibodies or antibody fragments purified using CaptureSelect IgG-CH1 Affinity Matrix.

Assay ELISA
Content And Storage Store in freezer (-5°C to -30°C).
Format Kit
For Use With (Equipment) Microplate Reader