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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (4,058)
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ELISA Substrates and Reagents (82)

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1

Invitrogen™ Human VEGF ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Human
Conjugate FAM
Incubation Time 1 hr.
Total Assay Time 2 hr
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.3 pg/mL
Storage Requirements -20°C
Assay Range 0.32-25000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 min
Detection Method Taqman™ Real-time PCR
Accession Number P15692
Regulatory Status RUO
Target VEGF
Kit Contents Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 7422
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
2
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Invitrogen™ Mouse 5'-Nucleotidase/CD73 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10%
Gene Symbol NT5E
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 7 pg/mL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 7-1500 pg/mL
Test Time 1 hr 20 min
Accession Number Q61503
Regulatory Status RUO
Target CD73
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 23959
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
3

Invitrogen™ Mouse Epiregulin (EREG) ELISA Kit  DFS Item

ELISA

Protein Family Growth Factors & Receptors
Target Species Mouse
Conjugate HRP
Intraassay CV <10%
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 10 pg/mL
Research Discipline Angiogenesis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr 20 min
Assay Solid-phase sandwich ELISA
Target Epiregulin
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Label or Dye Biotin
Calibration Range 10 pg/mL to 6000 pg/mL
Interassay CV <12%
Gene Symbol Ereg
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 10-6000 pg/mL
Accession Number Q61521
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 13874
Sample Type Plasma,Serum,Supernatant
Synonym EPR
4

Invitrogen™ Human Thrombospondin 1 ELISA Kit  DFS Item

ELISA

Protein Family Growth Factors & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10%
Total Assay Time 4 hr 45 min
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 100 μL
Assay Sensitivity 6 ng/mL
Research Discipline Cardiovascular Research
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr 20 min
Assay Solid-phase sandwich ELISA
Target Thrombospondin 1
Specificity This ELISA pair antibody detects human Thrombospondin-1. It shows less than 5% cross-reactivity with recombinant human Thrombospondin-2 and recombinant human Thrombospondin-4.
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Label or Dye Biotin
Calibration Range 6 ng/mL to 2000 ng/mL
Interassay CV <12%
Gene Symbol THBS1
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 6-2000 ng/mL
Accession Number P07996
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 7057
Sample Type Plasma,Serum,Supernatant
Synonym THBS, THBS-1, TSP, TSP-1, TSP1
5

Invitrogen™ Human TGFBR2 ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10%
Total Assay Time 4 hr 45 min
Sample Volume Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
Assay Sensitivity 15 pg/mL
Research Discipline Apoptosis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr 20 min
Assay Solid-phase sandwich ELISA
Target TGFBR2
Specificity This ELISA antibody pair detects human TGF-beta R2. Other species not determined yet.
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Label or Dye Biotin
Calibration Range 1.5 pg/mL to 500 pg/mL
Interassay CV <12%
Gene Symbol TGFBR2
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 1.5-500 pg/mL
Accession Number P37173
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 7048
Sample Type Plasma,Serum,Supernatant
Synonym AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, TGFbeta-RII
6

Invitrogen™ Mouse CTLA-4 ELISA Kit  DFS Item

ELISA

Protein Family Chemokines & Receptors
Host Species Mouse
Target Species Mouse
Conjugate HRP
Intraassay CV <10%
Detectable Analytes CTLA-4
Substrate Type TMB
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 5 pg/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr 20 min
Detection Method Colorimetric
Assay Solid-phase sandwich ELISA
Target CTLA-4 (CD152)
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL- 17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Label or Dye Biotin
Calibration Range 5 pg/mL to 1200 pg/mL
Interassay CV <12%
Gene Symbol CTLA4
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 5-1200 pg/mL
Accession Number P09793
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 12477
Sample Type Plasma,Serum,Supernatant
Synonym CH29-120A16.2, Cd152, Ctla-4, Ly-56
7
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Invitrogen™ Human TGF-beta RIII ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10%
Gene Symbol TGFBR3
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 40 pg/mL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 40-10000 pg/mL
Test Time 1 hr 20 min
Accession Number Q03167
Regulatory Status RUO
Target TGFBR3
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 7049
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
8

Invitrogen™ Human CCL3 (MIP-1 alpha) Uncoated ELISA Kit  DFS Item

ELISA

Target Species Human
Conjugate HRP
Gene Symbol Ccl3
Total Assay Time 24 hr 30 min
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 31.25 pg/mL
Storage Requirements 2-8°C
Assay Range 31.25-2000 pg/mL
Test Time 1 hr 45 min
Accession Number P10147
Regulatory Status RUO
Target CCL3 (MIP-1 alpha)
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Streptavidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Streptavidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 6348
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
9
Promotions Available

Invitrogen™ Human Kynureninase ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10%
Gene Symbol Kynu
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.61 ng/mL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 0.61-150 ng/mL
Test Time 1 hr 20 min
Accession Number Q16719
Regulatory Status RUO
Target KYNU
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 8942
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
10

Invitrogen™ Human S100 A6 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10%
Gene Symbol S100A6
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.06 ng/mL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 0.066-16 ng/mL
Test Time 1 hr 20 min
Accession Number P06703
Regulatory Status RUO
Target S100A6
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 6277
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
11
Promotions Available

Invitrogen™ Human Thrombospondin-2 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10%
Gene Symbol THBS2
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.68 ng/mL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 0.68-160 ng/mL
Test Time 1 hr 20 min
Accession Number P35442
Regulatory Status RUO
Target Thrombospondin 2
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 7058
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
12
Promotions Available

Invitrogen™ Human IDUA ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10%
Gene Symbol IDUA
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.16 ng/mL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 0.16-40 ng/mL
Test Time 1 hr 20 min
Accession Number P35475
Regulatory Status RUO
Target IDUA
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3425
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
13

Invitrogen™ Human Vitronectin (VTN) ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Target Species Human
Conjugate HRP
Intraassay CV <10%
Total Assay Time 4 hr 45 min
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Assay Sensitivity 0.8 ng/mL
Research Discipline Cardiovascular Research
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr 20 min
Assay Solid-phase sandwich ELISA
Target Vitronectin
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human A2M, ApoA1, ApoC1, ApoC2, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin D, Ck beta 8-1 , CNTF, EMMPRIN, Endostatin, Fibrinogen, FLRG, GITR Ligand, GPBB, Hemoglobin, HTRA2, IL-17 E, IL-1R3, Lactoferrin, Mesothelin, PAPP-A, PDECGF, PDGF Ralpha, Pepsinogen II, Periostin, Persephin, PTH, RAP, SAA, Serpin F1, Tie-1, tPA, TSP-2, Ubiquitin+1, Vitronectin, vWF
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Label or Dye Biotin
Calibration Range 0.8 ng/mL to 200 ng/mL
Interassay CV <12%
Gene Symbol VTN
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 0.819-200 ng/mL
Accession Number P04004
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 7448
Sample Type Plasma,Serum,Supernatant
Synonym V75, VN, VNT
14

Invitrogen™ Human Hemoglobin ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10%
Gene Symbol HBA1, HBB
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 1.5-400 ng/mL
Test Time 1 hr 20 min
Accession Number P68871, P69905
Regulatory Status RUO
Target Hemoglobin
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3039, 3043
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
15

Invitrogen™ Rat IgM Uncoated ELISA Kit with Plates  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 299357
Assay Sensitivity 0.3125 ng/ml
Assay Range 0.3125 to 20 ng/mL