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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 933 results
1

Thermo Scientific™ Acclaim™ VANQUISH™ Polar Advantage II UHPLC Columns

Achieve even greater power of separation, speed, and throughput of polar and nonpolar compounds in a single run.

Pore Size 120 Å
Stationary Phase Polar Embedded
Temperature 60°C
Phase Reversed Phase
For Use With LC/MS and RSLC
Endcapped Yes
Column Type Reversed Phase
Surface Area 300 m²/g
Max. Pressure 21756 psi (1500 bar)
Column Format Analytical Column
Particle Size 2.2 μm
Carbon Load 17%
Packing Material Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric) 2.1 mm
pH 1.5 to 10
Technique Bio-LC
Product Line Acclaim
USP Type L60
2

Thermo Scientific™ Hypersil™ MOS-2 C8 HPLC Columns

Use as a general-purpose C8 phase for acidic and neutral analytes, with decrease silanol activity

Pore Size 120 Å
Carbon Load 6.5%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L7
3

Thermo Scientific™ Hypersil™ SAS C1 HPLC Columns

Use for moderately polar and multifunctional compound selectivity.

Pore Size 120 Å
Stationary Phase C1
Temperature 60°C
pH 2 to 8
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L13
4

Thermo Scientific™ Hypersil™ Phenyl HPLC Columns

Use as a general-purpose column with aromatic selectivity.

Pore Size 120 Å
Carbon Load 5%
Temperature 60°C
Phase Reversed Phase
Endcapped No
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L11
5

Thermo Scientific™ Hypersil™ ODS (C18) Columns, 10μm Particle Size

Provide an excellent C18 phase for a broad range of applications and a global standard for many existing methods

Pore Size 120 Å
Particle Size 10 μm
Carbon Load 10%
Stationary Phase C18
Phase Reversed Phase
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
6

Thermo Scientific™ Hypersil™ MOS C8 HPLC Columns

Use as a general-purpose C8 phase for acidic and neutral analytes.

Pore Size 120 Å
Carbon Load 6.5%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L7
8

MilliporeSigma™ Supelco™ SRT™-C SEC-100 Gel Filtration Column, 5 μm Particle Size, PEEK

The bonded phase in SRT™-C SEC columns is more polar than the hydrophilic phase used in SRT™ columns, making SRT™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.

9

Thermo Scientific™ Hypersil™ APS-2 Columns, 3μm and 5μm Particle Size

Versatile amino propyl phase with excellent chromatographic properties in three modes: weak anion exchange, reversed phase and normal phase

Pore Size 120 Å
Particle Size 3 μm
Carbon Load 2%
Stationary Phase Amino (NH2)
Temperature 60°C
Phase Reversed Phase, Normal Phase, Ion Exchange, HILIC
Endcapped No
Column Type Mixed Mode
pH 2 to 8
Surface Area 170 m²/g
USP Type L8
10

MilliporeSigma™ Supelco™ ChromegaChiral™ Chiral CCA HPLC Column, 3 μm Particle Size

A polysaccharide coated chiral stationary phase and columns which are produced using a unique production process of coating the proven chiral selector, tris-(3,5-di- methylphenyl) carbamoyl amylose on high purity silica gel.

Pore Size 1000 Å
Particle Size 3 μm
Stationary Phase carbamoyl
Phase Chiral
Matrix carbamoyl Phase
Technique Chiral HPLC
Product Line ChromegaChiral
11

Thermo Scientific™ Accucore™ Biphenyl Reversed Phase HPLC Columns

Use this solid core column for fast and reliable separation of critical pairs and isomers.

Pore Size 80 Å
Stationary Phase Biphenyl
Temperature 70°C
Phase Reversed Phase
For Use With UHPLC
Endcapped Yes
Surface Area 130 m²/g
Max. Pressure 8700 psi (600 bar)
Particle Size 2.6 μm
Carbon Load 6%
Packing Material Ultrapure Silica
Diameter (Metric) 2.1 mm
pH 2 to 8
Particle Shape Solid Core
Product Line Accucore
USP Type L11
12

Thermo Scientific™ Acclaim™ Mixed Mode HILIC-1 HPLC Columns
GREENER_CHOICE

Get all the advantages of reversed-phase and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1 column.

Pore Size 120 Å
Stationary Phase Hydrophobic Alkyl Chain with a Diol Group
Temperature 50°C
Phase Reversed Phase and HILIC
Endcapped Proprietary
Column Type Mixed Mode
pH 2.5 to 7.5
Green Features FSC
Surface Area 300 m²/g
Product Line Acclaim
USP Type N/A
13

Thermo Scientific™ ProPac™ PA1 HPLC Columns

Efficiently analyze and purify hydrophilic anionic proteins and peptides with ProPac™ PA1 LC Columns.

Pore Size Nonporous
Particle Size 10 μm
Flow Rate 0.9 to 1.5 mL/min
Stationary Phase Alkyl Quaternary Amine
Phase Ion Exchange
For Use With BioLC Chromatography System
Packing Material Spherical, Nonporous Polymer
Column Type Ion Exchange
Quantity Each
Green Features FSC
Material PEEK
Max. Pressure 5000 psi (345 bar)
Product Line ProPac
14

Thermo Scientific™ ProPac™ SCX-10 HPLC Columns
GREENER_CHOICE

Separate protein variants in difficult matrices using ProPac SCX-10 LC Columns, which provide exceptionally high resolution.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Sulfonate
Temperature 60°C
Phase Ion Exchange
Column Type Ion Exchange
pH 2 to 12
Product Line ProPac
15

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns
GREENER_CHOICE

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pore Size 120 Å
Carbon Load 8%
Stationary Phase Proprietary
Temperature 70°C
Phase HILIC
For Use With LC/MS
Endcapped Yes
Column Type HILIC
pH 2 to 8
Surface Area 300 m²/g
Product Line Acclaim