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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 928 results
1

MilliporeSigma™ Supelco™ Ascentis™ Express Phenyl-Hexyl HPLC Column, 2.7 μm Particle Size

Ascentis™ Express Phenyl-Hexyl phase columns have unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Phenyl-Hexyl
Phase Reversed Phase
Matrix Phenyl-Hexyl Phase/Fused-Core Particle Platform
Endcapped Yes
Technique Fused-Core HPLC
Product Line Ascentis
2

MilliporeSigma™ Supelco™ Antibodix™ WCX-NP3 Weak Cation Exchange Column, 3 μm Particle Size, Stainless Steel

Antibodix™ columns are designed for the reproducible analysis of charge variants of monoclonal antibodies by salt or pH gradient.

Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Spherical PS-DVB
Phase Cation Exchange
Matrix Spherical PS-DVB Phase
pH 2.0 to 12.0
Material Stainless Steel
Technique Cation Exchange
Product Line Antibodix
3

Thermo Scientific™ SurePac™ Bio 550 SEC MDi™ HPLC Columns

Experience the future of Size-Exclusion Chromatography (SEC) with SurePac™ Bio 550 SEC MDi™ analytical columns and guards exclusively designed to deliver robust and reproducible performance at high resolution and throughput to accelerate your therapeutics analysis process.

Particle Size 3 μm
Stationary Phase Proprietary
Temperature 20°C to 60°C
Phase Size Exclusion
Packing Material Monodisperse Silica Resin
pH 2 to 8
Quantity 1 Ea.
Technique SEC
4

MilliporeSigma™ Supelco™ Ascentis™ Express OH5 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express OH5 columns can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Hydroxy, Penta-
Phase Reversed Phase
Matrix Hydroxy, Penta- Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
5

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 3 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pore Size 300 Å
Particle Size 3 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/silica particle platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
6

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 5 μm Particle Size

Ascentis™ Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase PFP (Pentafluorophenyl)
Phase Reversed Phase
Matrix PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 1 to 9
Technique Fused-Core HPLC
Product Line Ascentis
7

Thermo Scientific™ Hypersil™ BDS Phenyl (Ph) Reversed Phase HPLC Column, 5 μm, 4 mm x 300 mm

Column Format: Analytical Column; Carbon Load: 5%; Pore Size: 130 Å; Column Phase: Reversed Phase

8

Thermo Scientific™ Hypersil GOLD™ VANQUISH™ aQ UHPLC Columns

Use for UHPLC high pressure applications to retain and resolve polar analytes with highly aqueous mobile phases on the Vanquish UHPLC system.

Pore Size 175 Å
Stationary Phase aQ C18
Temperature 60°C
Phase Reversed Phase
For Use With UHPLC
Endcapped Polar
Surface Area 220 m²/g
Max. Pressure 18130 psi (1250 bar)
Column Format Analytical Column
Particle Size 1.9 μm
Carbon Load 12%
Packing Material Ultrapure Silica
Diameter (Metric) 2.1 mm
pH 2 to 9
Product Line Hypersil GOLD
USP Type L1
9

MilliporeSigma™ Supelco™ Carbopack X™ Thermal Desorption Tubes

Suitable for use with Air Monitoring

Type Thermal Desorption Tube
Matrix Carbopack™ x Graphitized Carbon Black (GCB)
For Use With (Equipment) PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer 1/4 in.
Packaging Quantity 10/Pk.
Material Glass
Overall Length (English) 3.5 in.
For Use With (Application) Air Monitoring
Methods EPA TO-17
Product Line Carbopack
10

MilliporeSigma™ Supelco™ Graphsphere™ 2016 Glass Thermal Desorption Tubes

Fritted and Preconditioned TD Tubes

Type Thermal Desorption Tube
For Use With (Equipment) PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer 1/4 in.
Material Glass
Overall Length (English) 3.5 in.
For Use With (Application) An Alternative to Tenax™ TA
11

MilliporeSigma™ Supelco™ TSKgel™ GMHHR-H(30) HT2 HPLC Column, 30 μm Particle Size, Stainless Steel

TSKgel™ Ultra-High Temperature columns are for the analysis of organic-soluble polymers at extremely elevated temperatures.

12

TSKgel™ Octadecyl-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

The highly cross-linked, polymethacrylate base material of TSKgel Octadecyl-NPR provides excellent stability in high pH buffer systems. It can withstand rigorous cleaning with either acid or base.

13

Thermo Scientific™ BetaSil™ C6 HPLC Columns, 5μm Particle Size

High surface area with high bonded phase coverage

Pore Size 100 Å
Particle Size 5 μm
Carbon Load 11%
Stationary Phase C6
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
USP Type L15
Column Format Analytical Column
14

Thermo Scientific™ Hypersil GOLD™ Amino HPLC Columns

Achieve outstanding peak shape and sensitivity in weak anion exchange, reversed phase and normal phase modes with Hypersil GOLD Amino LC Columns.

Pore Size 175 Å
Carbon Load 2%
Stationary Phase Amino (NH2)
Temperature 60°C
Phase Reversed Phase, Normal Phase, Ion Exchange, HILIC
Endcapped Yes
pH 2 to 8
Surface Area 220 m²/g
USP Type L8
15

Thermo Scientific™ Hypersil GOLD™ AX HPLC Columns

Efficiently separate smaller proteins, peptides, anionic species and polar molecules with Thermo Scientific™ Hypersil GOLD™ AX LC Columns.

Pore Size 175 Å
Carbon Load 6%
Stationary Phase Polymeric Amine
Temperature 60°C
Phase HILIC and Ion Exchange
Endcapped No
pH 2 to 8
Surface Area 220 m²/g