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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Keyword Search:
1 – 15 of 933 results
1

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

2

Thermo Scientific™ OLIGO™ R3 Reversed Phase Columns

Thermo Scientific™ Oligo™ R3 bulk resin is a rigid polymeric bead designed for reversed-phase chromatography of polynucleotides and other biomolecules.

Shipping Condition Room Temperature
Pore Size 350 Å
Content And Storage Store at room temperature.
Stationary Phase Phenyl
Length (Metric) 100 mm
Column Type Reversed Phase
Volume (Metric) 1.7 mL
Dimensions 4.6 x 100 mm
Quantity 1 Column
Max. Pressure 2400 psi (170 bar)
Column Format Prepacked Column
Particle Size 30 μm
Type Analytical Column
Diameter (Metric) 4.6 mm
Product Line POROS
3

Thermo Scientific™ Acclaim™ 300 C18 HPLC Columns
GREENER_CHOICE

Use these wide-pore C18 columns to achieve high-resolution protein separations.

Pore Size 300 Å
Stationary Phase C18
Temperature 60°C
For Use With LC/MS
Endcapped Yes
Column Type Reversed Phase
Green Features FSC
Surface Area 100 m²/g
Particle Size 3 μm
Flow Rate 1 mL/min
Carbon Load 8%
pH 2.5 to 7.5
Product Line Acclaim
USP Type L1
4

Thermo Scientific™ Acclaim™ Trinity P1 HPLC Columns
GREENER_CHOICE

Use these mixed-mode columns for simultaneous separation of pharmaceutical drug substances and their counter ions with high efficiency.

Pore Size 300 Å
Particle Size 3 μm
Stationary Phase Reversed Phase/Anion Exchange/Cation Exchange
Temperature 45°C
Phase Reversed Phase and Ion Exchange
Endcapped Proprietary
Column Type Mixed Mode
pH 2.5 to 7
Green Features FSC
Surface Area 100 m²/g
Max. Pressure 4500 psi (310 bar)
Product Line Acclaim
5

MilliporeSigma™ Supelco™ Ascentis™ Express RP-Amide HPLC Column, 5 μm Particle Size

Ascentis™ Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis™ Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from 'phase collapse'.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped Yes
Technique Fused-Core HPLC
Product Line Ascentis
6

MilliporeSigma™ Supelco™ Discovery™ RP-AmideC16 HPLC Column, 5 μm Particle Size

Discovery™ RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.

Pore Size 180 Å
Particle Size 5 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Silica Gel, High Purity, Spherical Base Material
Endcapped Yes
pH 2 to 8
Surface Area 200 m2/g
Product Line Discovery
7

Thermo Scientific™ Hypersil™ PREP HS C18 HPLC Columns

Maximize retention and loading with Hypersil PREP HS C18 prep LC reversed phase columns which offers high loading capacity and suitable for USP-L1 Applications.

Pore Size 100 Å
Carbon Load 15%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 9
Surface Area 350 m²/g
USP Type L1
8

MilliporeSigma™ Supelco™ LiChrospher™ NH2 HPLC Column, 5 μm Particle Size

LiChrospher™ is a reliable and versatile spherical silica sorbent, which is traditionally produced and available with different modifications.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase Amino (NH2)
Phase Normal Phase, HLIC, Anion Exchange
Matrix Amino (NH2) Phase
Endcapped No
Surface Area 350 m2/g
Product Line LiChrospher
9

Thermo Scientific™ HyPURITY™ ADVANCE™ HPLC Columns, 5μm Particle Size

Use this specialty C8 column for separating compounds showing poor peak shape with traditional alkyl bonded phases.

Particle Size 5 μm
Carbon Load 9%
Stationary Phase C8
Phase Reversed Phase
Packing Material Silica
Column Type Reversed Phase
Surface Area 200 m²/g
USP Type L7
10

MilliporeSigma™ Supelco™ Proteomix™ SAX-NP10 Strong Anion Exchange Column, 10 μm Particle Size, PEEK

Used for the analyis or small scale purification of negatively charged biopolymers, such as glycoprotein isoforms, pegylated proteins and oligonucleotide.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Ammonium Ion, Quaternary
Phase Anion Exchange
Matrix Spherical PS-DVB/Ammonium Ion, Quaternary Phase
pH 2.0 to 12.0
Material PEEK
Technique Anion Exchange HPLC
Product Line Proteomix
11

MilliporeSigma™ Supelco™ Antibodix™ WCX-NP10 Weak Cation Exchange Column, 10 μm Particle Size, PEEK

Antibodix™ columns are designed for the reproducible analysis of charge variants of monoclonal antibodies by salt or pH gradient.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Spherical PS-DVB
Phase Cation Exchange
Matrix Spherical PS-DVB Phase
pH 2.0 to 12.0
Material PEEK
Technique Cation Exchange
Product Line Antibodix
12

TSKgel™ G4000PWXL HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ columns may be used for the analysis, isolation and purification of biomolecules.

13

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-SCX HPLC Column, 5 μm Particle Size

The LC-SCX cation-exchange columns have strongly acidic propylsulfonic acid groups and are used for separating cations. Adjust pH, ionic strength, and organic modifier concentration to control retention and selectivity.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase Sulfonic Acid, Propyl-
Phase Cation Exchange
Matrix Sulfonic Acid, Propyl- Phase/Silica Gel, Spherical Particle Platform
Length (Metric) 25 cm
Surface Area 170 m2/g
Product Line SUPELCOSIL
14

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
15

TSKgel™ G4000SWXL HPLC Column, 8 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel™ SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.