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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 933 results
1

TSKgel™ Amide-80 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase Carbamoyl
Temperature 10°C to 50°C
Max. Pressure 200 bar
Product Line TSKgel
USP Type L68
2

Thermo Scientific™ Acclaim™ VANQUISH™ Polar Advantage II UHPLC Columns

Achieve even greater power of separation, speed, and throughput of polar and nonpolar compounds in a single run.

Pore Size 120 Å
Stationary Phase Polar Embedded
Temperature 60°C
Phase Reversed Phase
For Use With LC/MS and RSLC
Endcapped Yes
Column Type Reversed Phase
Surface Area 300 m²/g
Max. Pressure 21756 psi (1500 bar)
Column Format Analytical Column
Particle Size 2.2 μm
Carbon Load 17%
Packing Material Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric) 2.1 mm
pH 1.5 to 10
Technique Bio-LC
Product Line Acclaim
USP Type L60
3

Thermo Scientific™ Hypersil™ Phenyl HPLC Columns

Use as a general-purpose column with aromatic selectivity.

Pore Size 120 Å
Carbon Load 5%
Temperature 60°C
Phase Reversed Phase
Endcapped No
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L11
4

MilliporeSigma™ Supelco™ ChromegaChiral™ Chiral CC4 HPLC Column, 5 μm Particle Size

ChromegaChiral™ CC4 (cellulose tris(4-chloro-3-methyl-phenylcarbamate) is another new product for high resolution chiral separations based on a new halogenated carbohydrate based chiral stationary phase.

Pore Size 1000 Å
Particle Size 5 μm
Stationary Phase C4 (Butyl)
Phase Chiral
Matrix C4 (Butyl) Phase
Technique Chiral HPLC
Product Line ChromegaChiral
5

TSKgel™ ODS-80Ts HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

A general purpose column for low MW pharmaceuticals, bases, nucleosides, and nucleotides.

6

Thermo Scientific™ μPAC™ Trapping Column, 2/pk

Perform large volume (>5 μL) sample injections on capillary and nano-columns with μPAC™ Trapping Columns.

7

Thermo Scientific™ GlycanPac™ AXR-1 HPLC Columns
GREENER_CHOICE

Get ultra-high resolution and selectivity for both labeled and native glycans using this HPLC/UHPLC column. It's compatible with MS and fluorescence detection.

Pore Size 175 Å
Stationary Phase Proprietary
Temperature 60°C
Phase Reversed Phase and Ion Exchange
Column Type Mixed Mode
pH 2 to 8
Product Line GlycanPac
8

Thermo Scientific™ BetaSil™ C18 HPLC Columns, 3μm Particle Size

High surface area with high bonded phase coverage

Pore Size 100 Å
Particle Size 3 μm
Carbon Load 20%
Stationary Phase C18
Phase Reversed Phase
Column Type Reversed Phase
USP Type L1
9

Thermo Scientific™ BetaBasic™ 18 HPLC Columns, 5μm Particle Size

Available in limited dimensions. Please contact Customer Service for more details.

Pore Size 150 Å
Particle Size 5 μm
Carbon Load 13%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS, APCI
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 12
Surface Area 200 m²/g
USP Type L1
Column Format Analytical Column
10

Thermo Scientific™ BetaSil™ Phenyl/Hexyl Columns, 5μm Particle Size

Thermo ScientificTM BetaSilTM Phenyl/Hexyl Columns are available in limited dimensions. Please contact Customer Service for more details.

Pore Size 100 Å
Stationary Phase Phenyl/Hexyl
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Column Type Reversed Phase
Column Format Analytical Column
Particle Size 5 μm
Carbon Load 11%
Packing Material Silica
Diameter (Metric) 4.6 mm
pH 2 to 9
Product Line Beta
USP Type L11
11

Thermo Scientific™ Accucore™ C18 HPLC Columns

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

Pore Size 80 Å
Particle Size 2.6 μm
Carbon Load 9%
Stationary Phase C18
Temperature 70°C
Phase Reversed Phase
Endcapped Yes
pH 1 to 11
Surface Area 130 m²/g
Particle Shape Solid Core
USP Type L1
12

MilliporeSigma™ Supelco™ Zenix™-C SEC-150 Gel Filtration Column, 3 μm Particle Size, Stainless Steel

The bonded phase in Zenix™-C SEC columns is more polar than the hydrophilic phase used in Zenix™ columns, making Zenix™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.

Pore Size 150 Å
Particle Size 3 μm
Stationary Phase Hydrophilic Polymer
Phase Size Exclusion
Matrix Hydrophilic Polymer Phase/Spherical Silica
Length (Metric) 30 cm
pH 2.0 to 8.5
Material Stainless Steel
Technique SEC HPLC
Product Line Zenix
13

MilliporeSigma™ Supelco™ ChromegaChiral™ Chiral CCO F4 HPLC Column, 5 μm Particle Size

ChromegaChiral™ CCO F4 is a 4-Fluoro 3-methylphenyl cellulose phase which can be used in SFC or HPLC.

Pore Size 1000 Å
Particle Size 5 μm
Stationary Phase 2-fluoro 5-methylphenyl cellulose
Phase Chiral
Matrix 2-fluoro 5-methylphenyl cellulose Phase
Technique Chiral HPLC
Product Line ChromegaChiral
14

MilliporeSigma™ Supelco™ ChromegaChiral™ Chiral CC3 HPLC Column, 5 μm Particle Size

ChromegaChiral™ CC3 (amylose tris(5-chloro-2-methyl- phenylcarbamate) is a new product for high resolution chiral separations based on a new halogenated carbohy- drate based chiral stationary phase.

Pore Size 1000 Å
Particle Size 5 μm
Stationary Phase Amylose, carbamate
Phase Chiral
Matrix Amylose, carbamate Phase
Technique Chiral HPLC
Product Line ChromegaChiral
15

MilliporeSigma™ Supelco™ Proteomix™ SAX-NP1.7 Strong Anion Exchange Column, 1.7 μm Particle Size, Stainless Steel

Used for the analyis or small scale purification of negatively charged biopolymers, such as glycoprotein isoforms, pegylated proteins and oligonucleotide.

Pore Size Nonporous
Particle Size 1.7 μm
Stationary Phase Ammonium Ion, Quaternary
Phase Anion Exchange
Matrix Spherical PS-DVB/Ammonium Ion, Quaternary Phase
pH 2.0 to 12.0
Material Stainless Steel
Technique Anion Exchange HPLC
Product Line Proteomix