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Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)
Description
- A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency
- Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
- Maintains maximum on-target editing efficiency compared to TrueCut™ Cas9 Protein v2
- Transfection-ready using either electroporation or lipid-mediated transfection reagents
- Manufactured under strict ISO-13485 quality standards
- Ideal for applications that are sensitive to off-target events and when more precise editing is required
- Eliminates time consuming cloning steps
Available in Two Concentrations
- 1 μg/μL - For use in standard editing scenarios
- 5 μg/μL - For optimized editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
- For custom sizes or concentrations, please inquire
Specifications
Specifications
| Concentration | 1 μg/μL |
| Purity | ≥90% |
| Sensitive to Heat Inactivation | Yes |
| Source | E. coli |
| Quantity | 25 μg |
| Product Type | HiFi Cas9 |
| Form | Liquid |
Frequently Asked Questions (FAQs)
Both Gibco CTS HiFi Cas9 Protein (Cat. No. A54223, A54224) and Invitrogen TrueCut HiFi Cas9 Protein (Cat. No. A50574, A50575) are Streptococcus pyogenes high-fidelity CRISPR Cas9 proteins, produced by recombinant DNA technology in E. coli. Both proteins have the same sequence with added nuclear localization signal. Gibco CTS HiFi Cas9 Protein is manufactured to meet the standards for “Ancillary Materials for Cell, Gene, and Tissue-Based Products” whereas Invitrogen TrueCut HiFI Cas9 Protein is a research-grade product.
Yes, we offer TrueCut HiFi Cas9 Protein in the following concentrations:
1 µg/µL (Cat. Nos. A50574, A50575) for use in standard editing scenarios
5 µg/µL (Cat. Nos. A50576, A50577) for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
TrueCut HiFi Cas9 Protein is a modified Cas9 enzyme engineered to exhibit superior off-target profile, while maintaining maximum on-target cleavage efficiency of the wild type TrueCut Cas9 Protein v2. TrueCut HiFi Cas9 Protein is ideal for experiments that are sensitive to off-target events while still requiring a high level of editing efficiency.
The storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein is as follows:
15 mM Tris-HCl pH 8.0, 150 mM NaCl, 1.0 mM TCEP, 50% glycerol.
We recommend using Lipofectamine CRISPRMAX Transfection Reagent with TrueCut Cas9 Protein v2 or TrueCut HiFi Cas9 Protein.