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Thermo Scientific™ T4 beta-glucosyltransferase
Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis
Supplier: Thermo Scientific™ EO0831
Description
T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating β-glucosyl-5 hydroxymethylcytosine.
Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1ωg DNA at 37°C in 15 minutes.
Highlights
- Specific – selectively transfers glucose to the hydroxymethyl moiety of 5-hmC
- Fast – complete glucosylation of 1μg DNA in just 15 min
- Convenient – supplied with optimized buffer and UDP-glucose
- Locus specific detection of 5-hmC
- Enrichment of 5-hmC containing DNA
- Labeling of 5-hmC residues using a radioactive UDP-glucose donor
- T4 β-glucosyltransferase, 5 U/μL
- 10X Epi buffer
- 10X UDP-glucose
Specifications
| T4 β-glucosyltransferase | |
| 10X Epi Buffer | |
| 500 units | |
| T4 Beta Glucosyltransferase |
For Research Use Only. Not for use in diagnostic procedures.