missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Équipement et fournitures pour PCR
  4. Fournitures pour PCR
  5. Produits de transcription inverse
  6. Transcriptases inverses
  7. Invitrogen™ SuperScript V Reverse Transcriptase

Invitrogen™ SuperScript V Reverse Transcriptase

Numéro de catalogue. 19090050
Modifier l'affichage
Click to view available options
Quantité:
10,000 Units
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. Quantité
19090050 10,000 Units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Numéro de catalogue. 19090050 Fournisseur Invitrogen™ Code fournisseur 19090050

Pour de l'aide SVP utiliser la function de clavardage; nous envoyer un couriel à help@thermofisher.com ou appelez le service à la clientèle au 1-800-234-7437.

Description

Invitrogen™ SuperScript™ V Reverse Transcriptase (RT) is a next-generation MMLV reverse transcriptase designed for fast, reliable first-strand cDNA synthesis.

Invitrogen™ SuperScript™ V Reverse Transcriptase (RT) is a next-generation MMLV reverse transcriptase designed for fast, reliable first-strand cDNA synthesis. Building on the advanced performance of the SuperScript™ IV reverse transcriptase, SuperScript V RT combines high sensitivity, thermostability, and processivity with improved resistance to oxidation and greater workflow flexibility.

SuperScript V RT supports reliable cDNA synthesis from low-input RNA as well as highly structured, GC-rich, degraded, and inhibitor-containing samples.

Features of SuperScript V Reverse Transcriptase include:

  • Broad RNA input range—supports cDNA synthesis from 100 fg to 5 μg of RNA
  • Robust performance with challenging RNA—reaction temperatures up to 65°C help transcribe highly structured and GC-rich RNA
  • Fast cDNA synthesis—complete first-strand cDNA synthesis in as little as 1–10 minutes
  • Improved resistance to oxidation— enhanced reagent stability supports ambient shipping and room-temperature storage for up to 6 months
  • Flexible workflow—room-temperature reaction setup and stability of assembled reactions for up to 24 hours

SuperScript V RT is well suited for RT-PCR, RT-qPCR, gene expression analysis, and other applications requiring high-quality first-strand cDNA synthesis. Its enhanced reagent and assembled-reaction stability provides additional flexibility for routine, high-throughput, and automated workflows.

A DTT-free, animal origin–free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.

Spécifications

Concentration 200 U/μL
Contenu et stockage • SuperScript V Reverse Transcriptase (50 μL)
• 5X SuperScript V Reaction Buffer (500 μL)

Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 12 months
• Room temperature for up to 6 months
Méthode de détection qPCR, NGS, PCR
Forme Liquid
Format Stand-alone Enzyme
Performance de la PCR pour les régions riches en GC High
Méthode PCR 2-step RT-qPCR, RT-PCR
Vitesse de réaction 10 min.
Technique Reverse Transcription
Étiquette ou colorant Not Applicable
Température de réaction optimale 50°C
Type de produit Reverse Transcription Reagent
Quantité 10,000 Units
Transcriptase inverse SuperScript V
Activité ribonucléase H Reduced
Sensibilité 0.1 pg total RNA
Shipping condition (Condition d'expédition) Dry Ice, Wet Ice, Ambient
À utiliser avec (application) cDNA Synthesis, RT-PCR, Real Time PCR (qPCR), Sequencing, Next-Generation Sequencing (NGS)
Thermostabitilé Up to 65°C
Nb de réactions 50 Reactions
Format de réaction Separate components
Type de réactif Reverse Transcription
Taille (produit final) Up to 20 kb
Matériau de départ RNA
Tolérance aux inhibiteurs (simple) High
Afficher plus de résultats Afficher moins de résultats

For Research Use Only. Not for use in diagnostic procedures.