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Gibco™ RevitaCell™ Supplement (100X)
Description
RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.
Enabling—provides maximum cell viability when performing single cell passaging
Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons
Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence
Single cell passaging
Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.
Higher specificity
Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).
Quicker post-thaw recovery
PSCs recovered at 40,000 viable cells/cm2 using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.
Single cell passaging
Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.
Higher specificity
Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).
Quicker post-thaw recovery
PSCs recovered at 40,000 viable cells/cm2 using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.
Specifications
Specifications
| Content And Storage | Store at -5 to -20°C and protect from light. |
| Cell Type | Stem Cells (iPS - Induced Pluripotent Stem) |
| Classification | Animal Origin-free |
| Concentration | 100 X |
| Culture Type | Stem Cell (Embryonic, iPS - Induced Pluripotent Stem) |
| Product Type | Media Supplement |
| Manufacturing Quality | cGMP for medical devices, 21 CFR Part 820 and ISO 13485 |
| Product Line | Gibco, RevitaCell |
| Quantity | 5 mL |
| Shipping Condition | Dry Ice |
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For Research Use Only. Not for use in diagnostic procedures.