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Thermo Scientific™ ProPac™ WCX-10 Carboxylate Ion Exchange HPLC Column, 10 μm, 4 mm x 50 mm
Description
Use Thermo Scientific™ ProPac™ WCX-10 LC Columns to provide exceptionally high resolution for separations of proteins and their variants. Based on stationary phases composed of 10μm nonporous polymeric beads, ProPac columns resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer grafted to the particle core eliminates unwanted secondary interactions. The cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.
Multiple Formats for Greater Flexibility
- ProPac WCX-10 column is a weak cation-exchanger with a carboxylate functional group
- Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semi-prep work
- Ideal for characterization and quality control assessment of MAbs and other proteins
- Well-suited for characterization of related protein variants including deamidation and MAb lysine truncation variants
- Unequaled high-resolution separations
- Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing
Specifications
Specifications
| Column Format | Analytical Column |
| Diameter (Metric) | 4 mm |
| Flow Rate | 1 mL/min |
| Length (Metric) | 50 mm |
| Max. Pressure | 3000 psi (207 bar) |
| Mobile Phase Compatibility | pH 2 to 12, 80% compatible with acetonitrile and acetone, compatible with nonionic and zwitterionic detergents, incompatible with cationic detergents |
| Particle Size | 10 μm |
| pH | 2 to 12 |
| Pore Size | Nonporous |
| Quantity | Each |
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