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Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns
Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.
Supplier: Thermo Scientific™ 063276
Description
Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.
Tailored Specificity for IMAC Separation of Proteins- Retention control by imidazole concentration or pH gradient
- Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
- Effective under native or denaturing HPLC conditions
- Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
- Shipped free from any metal and ready to be charged with the metal of your choice.
- Reusable columns with metal tailored specificity
- Resolve target proteins using a single column in a high resolution gradient run
- High purity separations of metal-binding proteins
Specifications
| Analytical Column | |
| 1 mL/min | |
| 50 mm | |
| pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl | |
| 2 to 12 | |
| Each | |
| Lysozyne Poly(IDA) Grafts | |
| Spherical, Nonporous Polymer | |
| Affinity Separation | |
| Bio-LC |
| 4 mm | |
| UltiMate 3000 HPLC System | |
| 3000 psi (207 bar) | |
| 10 μm | |
| Nonporous | |
| 60°C | |
| ProPac | |
| Separation of Metal-Binding Proteins | |
| Analytical Column | |
| Responsibly packaged |